HPLC Analysis of Aripiprazole and Dehydro Aripiprazole Using a Titan C18 Column

column

Titan C18, 10 cm x 3.0 mm I.D., 1.9 μm particles (577160-U)

mobile phase

[A] 10 mM ammonium formate; pH 3.0 with formic acid [B] 0.1% formic acid in methanol

gradient

40% B for 0.5 minutes followed by a linear gradient to 95% B for 3.5 minutes, followed by a hold at 95% B for 2 minutes.

flow rate

0.5 mL/min

pressure

6500 psi (445 bar)

column temp.

35 °C

detector

UV, 245 nm

injection

2 μL

sample

50 μg/mL in 10% methanol