Targeted genome editing has rapidly become a vital tool across the entire research continuum from early discovery to therapeutic application. By enabling scientists to selectively disrupt, recover, repress, or activate gene expression, modern gene editing methods allow for unprecedented exploration of genetic mechanisms governing biological processes. Application of genome engineering ranges from curing developmental disorders to developing disease-resistant crops.
Schematic diagram of the CRISPR gene editing system showing the different types of synthetic gRNA for CRISPR.
Gene editing is a specific and targeted change to a DNA sequence and involves the addition, removal or modification of the DNA. The CRISPR-Cas system (evolved in microbes as a defense mechanism) is the basis for a class of gene-editing tools that are enabling advances from health and diagnostics to agriculture and energy. Using CRISPR, researchers have the power to target a specific gene, gene family, or even screen an entire genome.
Sigma-Aldrich® Advanced Genomics offers an industry leading selection of CRISPR-Cas9 proteins to meet your individual research needs. Choose from multiple Cas9 variants (wild-type, enhanced specificity, nickase, GFP-fused, catalytically inactive) and several formats (plasmid, lentivirus, lyophilized protein).
CRISPRi (CRISPR interference) and CRISPRa (CRISPR activation) deliver highly efficient silencing and activation of genes, respectively, without altering the underlying DNA sequence. When employed in large scale LOF (loss-of-function) and GOF (gain-of-function) screens, researchers are able to identify unique, yet functionally related, gene pathways that are often missed with other methods.
Sigma-Aldrich® Advanced Genomics offers a complete suite of optimized CRISPRi and CRISPRa libraries for gene knockdown and overexpression experiments. Pooled CRISPRi and SAM CRISPRa lentiviral libraries are available off-the-shelf or customized to your specific needs.
Wondering if CRISPR is right for your project? Connect with a Sigma-Aldrich® Advanced Genomics Expert.