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06-863

Sigma-Aldrich

Anti-CREB Antibody

Upstate®, from rabbit

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Synonym(s):
Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1, CREB-1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

using Protein A

species reactivity

mouse, human, rat

species reactivity (predicted by homology)

porcine (based on 100% sequence homology), horse (based on 100% sequence homology), canine (based on 100% sequence homology)

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
electrophoretic mobility shift assay: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... CREB1(1385)

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This Item
AB300669050MAB5432
Anti-CREB Antibody Upstate®, from rabbit

06-863

Anti-CREB Antibody

Sigma-Aldrich

AB3006

Anti-CREB Antibody

Anti-Cre Antibody Novagen®

69050

Anti-Cre Antibody

Sigma-Aldrich

MAB5432

Anti-CREB Antibody

clone

polyclonal

clone

polyclonal

clone

-

clone

monoclonal

biological source

rabbit

biological source

rabbit

biological source

-

biological source

mouse

species reactivity

mouse, human, rat

species reactivity

human, mouse, rat

species reactivity

-

species reactivity

human, mouse, rat

UniProt accession no.

P16220

UniProt accession no.

P16220

UniProt accession no.

-

UniProt accession no.

P16220

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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General description

Cyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation by various kinases, including PKA, and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.

Specificity

This antibody recognizes CREB.

Immunogen

Epitope: Amino acids 5-24.
KLH-conjugated, synthetic peptide (CSGAENQ QSGDAAVTEAENQQ) corresponding to a.a. 5-24 of human CREB. The immunizing sequence is identical in pig, shares 19 of 20 residues with mouse and bovine and 18 of 20 residues with rat.

Application

Anti-CREB Antibody, Cat. No. 06-863, is a highly specific rabbit Polyclonal antibody, that targets CREB and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, Electrophoretic Mobility Shift Assay (EMSA), Immunoprecipitation, and Western Blotting.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated CREB in 500 µg of HeLa nuclear extract.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected an enhanced CREB occupancy at the LYPD3 promoter in LKB1-deficient TE-4 human esophageal cancer cells (Gu, Y., et al. (2012). Oncogene. 31(4):469–479).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB association at the RNASET2 sites targeted by T-cell Leukemia Virus type 1 (HTLV-1) encoded Tax protein using HTLV-1-infected human T-cell lines, MT-2, C8166/45 and SLB-1 (Polakowski, N., et al. (2011). Viruses. 3(8):1485-500).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB occupancy at the hBDNF promoter IV using postmortem human parietal cortex tissue chromatin preparations (Pruunsild, P., et al. (2011). J. Neurosci. 31(9):3295-3308).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB occupancy at the miR-9-2 promoter in SK-N-BE human neuroblastoma cells (Laneve, P., et al. (2010). Nucl. Acids Res. 38(20):6895-6905).
ChIP-seq Analysis: A representative lot identified CREB target genes in murine spermatogonia derived GC1-spg cells by ChIP-seq analysis (Martianov, I., et al. (2010). BMC Genomics. 11:530).
Western Blotting Analysis: Representative lots detected CREB in lysates from cultured embryonic rat striatal neurons and PC12 pheochromocytoma cells (Hutchinson, A.N., et al. (2012). Neuropsychopharmacology. 37(2):321-337; Maharjan, S., et al. (2010). J. Neurochem. 112(1):42-55).
Western Blotting Analysis: Representative lots detected CREB in mouse proximal caput epididymis-1 (PC-1) cell lysate and hippocampus tissue homogenates (Hamzeh, M., and Robaire, B. (2011). J. Endocrinol. 209(1):55-64; Zhou, Z., et al. (2009). Neuropharmacology. 56(1):81-89).
Western Blotting Analysis: A representative lot detected CREB in SK-N-BE human neuroblastoma cell lysate (Laneve, P., et al. (2010). Nucl. Acids Res. 38(20):6895-6905).
Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of DNA-CREB complex in EMSA using radiolabeled oligonucleotide containing hBDNF promoter I CREB-binding sequence and lysates from KCl-treated primary rat neurons (Pruunsild, P., et al. (2011). J. Neurosci. 31(9):3295-3308).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CREB in 10 µg of HeLa nuclear extract.

Target description

~43 kDa observed.

Linkage

Replaces: 04-218

Physical form

Protein A purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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Activating transcription factor 3 (ATF3) represses the expression of CCL4 in murine macrophages.
Khuu, CH; Barrozo, RM; Hai, T; Weinstein, SL
Molecular Immunology null
Neurofilament proteins and cAMP pathway in brains of mu-, delta- or kappa-opioid receptor gene knock-out mice: effects of chronic morphine administration.
J A Garcia-Sevilla, M Ferrer-Alcon, M Martin, B L Kieffer, R Maldonado
Neuropharmacology null
Hippocampal overexpression of mutant creb blocks long-term, but not short-term memory for a socially transmitted food preference.
Brightwell, JJ; Smith, CA; Countryman, RA; Neve, RL; Colombo, PJ
Learning & Memory null
Serban San-Marina et al.
Biochimica et biophysica acta, 1783(3), 503-517 (2007-12-28)
The basic helix-loop-helix (bHLH) transcription factor family contains key regulators of cellular proliferation and differentiation as well as the suspected oncoproteins Tal1 and Lyl1. Tal1 and Lyl1 are aberrantly over-expressed in leukemia as a result of chromosomal translocations, or other
C Sala et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 20(10), 3529-3536 (2000-05-11)
Developmental changes in the signaling properties of NMDA receptors have been proposed to underlie the loss of plasticity that accompanies brain maturation. Calcium influx through postsynaptic NMDA receptors can stimulate neuronal gene expression via signaling pathways such as the Ras-MAP

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