322123

5,5′-Dithio-bis-(2-nitrobenzoic Acid)

Sulfhydryl reagent used to characterize reactive SH groups.

• Synonym(s): 5,5′-Dithio-bis-(2-nitrobenzoic Acid), DTNB, Ellman’s Reagent
• Empirical Formula (Hill Notation): C₁₄H₈N₂O₈S₂
• CAS Number: 69-78-3
• Molecular Weight: 396.35
• MDL number: MFCD00007140

Properties

• Quality Level: 100
• Assay: ≥98% (titration)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: yellow
• solubility: ethanol: 8 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)
• InChI key: KIUMMUBSPKGMOY-UHFFFAOYSA-N

Description

General description

Sulfhydryl reagent used to characterize reacting SH groups. Useful in photometric determination of thiols.

Sulfhydryl reagent used to characterize reactive SH groups. Useful in photometric determination of thiols.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Useful in photometric determination of thiols

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Preparation Note

May require gentle heating for complete solubilization.

Other Notes

Ellman, G.L., et al. 1961. Biochem. Pharmacol.7, 88.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3