RanBP-type and C3HC4-type zinc finger-containing protein 1 (UniProt Q9BYM8; also known as HOIL-1, HBV-associated factor 4, Heme-oxidized IRP2 ubiquitin ligase 1, Hepatitis B virus X-associated protein 4, RBCC protein interacting with PKC1, RING finger protein 54, Ubiquitin-conjugating enzyme 7-interacting protein 3) is encoded by the RBCK1 gene (also known as C20orf18, HOIL1, PBMEI, RBCK2, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4) (Gene ID 10616) in human. The linear ubiquitin assembly complex (LUBAC) is comprised of two RING-IBR-RING (RBR)-containing E3 ligases, HOIL-1L and HOIP. LUBAC conjugates a head-to-tail-linked linear polyubiquitin chain onto lysine residues on substrate proteins. Ovarian tumor deubiquitinating enzyme (DUB) with linear linkage specificity (OTULIN/Gumby/FAM105B) and cylindromatosis (CYLD) are two DUBs that catalyze the cleavage linear polyubiquitin chains. OTULIN and CYLD interact with the N-terminal PNGase/UBA or UBX (PUB) domain of HOIP and negatively regulate LUBAC-mediated cellular processes. LUBAC is reported to suppress IFN-dependent antiviral activity by mediating TRIM25 degradation and competing against TRIM25 for RIG-I interaction.
Clone 2E2 reacts with isoform 1 (HOIL-1L) and isoform 2, but not isoform 3.
Epitope: Near C-terminus
GST-tagged recombinant protein corresponding to human HOIL-1 near the C-terminus.
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-HOIL-1 Antibody, clone 2E2 is validated for use in Western Blotting, Immunocytochemistry for the detection of HOIL-1 .
Western Blotting Analysis: A representative lot detected HOIL-1 in HeLa, SH-SY5Y, and U20S cells (Kirisako, T., et al (2006). EMBO J. 25(20):48877-4887).
Western Blotting Analysis: A representative lot detected HOIL-1 in mouse liver extracts (Tokunaga, F., et al (2009). Nat. Cell Biol. 11(2):123-132).
Western Blotting Analysis: A representative lot detected HOIL-1 in MEFs (Takiuchi, T., et al (2014). Genes Cells. 19(3):254-272).
Immunocytochemistry Analysis: A representative lot detected HOIL-1 in HeLa cells with or without Sendai virus (SeV) infection (Inn, K.S., et al. (2011). Mol Cell. 41(3):354-365).
Evaluated by Western Blotting in THP-1 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected HOIL-1 in 10 µg of THP-1 cell lysate.
~57 kDa observed
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
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