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MABF2093

Sigma-Aldrich

Anti-SLAMF7 (CD319) Antibody, clone 162

clone 162, from mouse

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Synonym(s):
SLAM family member 7, CD2 subset 1, CD2-like receptor-activating cytotoxic cells, CRACC, Membrane protein FOAP-12, Novel Ly9, Protein 19A, CD319
eCl@ss:
32160702

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

162, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
inhibition assay: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... SLAMF7(57823)

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Anti-CRACC Antibody, clone 4G2 clone 4G2, from rat

MABF917

Anti-CRACC Antibody, clone 4G2

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified antibody

species reactivity

human

species reactivity

human

species reactivity

mouse

species reactivity

rat

biological source

mouse

biological source

rabbit

biological source

rat

biological source

rat

Gene Information

human ... SLAMF7(57823)

Gene Information

human ... SLAMF7(57823)

Gene Information

mouse ... Slamf7 (75345)

Gene Information

rat ... Klrb1C(683758)

UniProt accession no.

Q9NQ25

UniProt accession no.

Q9NQ25

UniProt accession no.

Q8BHK6

UniProt accession no.

Q5NKN4

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General description

SLAM family member 7 (UniProt: Q9NQ25; also known as SLAMF7, CD2 subset 1, CD2-like receptor-activating cytotoxic cells, CRACC, Membrane protein FOAP-12, Novel Ly9, Protein 19A, CD319) is encoded by the SLAMF7 (also known as CS1, UNQ576/PRO1138) gene (Gene ID: 57823) in human. SLAMF-7 is a single-pass type I membrane protein that is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression has also been detected in multiple myeloma cells, NK cells, activated B-cells, and NK-cell line, but not in promyelocytic, B-, or T-cell lines. It serves as a receptor on immune cells, including natural killer (NK) cells. SLAMF7 is reported to mediate both activating and inhibitory effects on NK cells, depending on the expression of adaptor EAT-2. In cells lacking EAT-2 it mediates inhibition via SHIP-1, which is recruited via Tyr 261 of SLAMF7. SLAMF7 is shown to be involved in phagocytosis of hematopoietic tumor cells independent of signaling lymphocyte activation molecule-associated protein (SAP) adaptors, but depends on its ability to interact with integrin Mac-1 and utilizes signals involving ITSM motif (aa 302-307). SLAMF7 is synthesized with a signal peptide (aa 1-22), which is subsequently removed to generate the mature form that has an extracellular domain (aa 23-226), a short transmembrane domain (aa 227-247), and a cytoplasmic tail (aa 248-335). Seven different isoforms of SLAMF7 have been described that are produced by alternative splicing. Clone 162 is shown to block the augmented capacity of human blood-derived macrophages to engulf Raji cells in response to Anti-CD47 antibodies. (Ref.: Guo, H., et al. (2015). Mol. Cell. Biol. 35 (1); 41-51; Chen, J., et al. (2017). Nature 544 (7651); 493-497).

Specificity

Clone 162 is a mouse monoclonal antibody that detects SLAM family member 7 (SLAMF7) in human cells. It targets an epitope with in the extracellular domain.

Immunogen

Epitope: extracellular domain
Recombinant protein fragment corresponding to 204 amino acids from the extracellular domain of human SLAM family member 7 fused with human IgG1 constant region.

Application

Anti-SLAMF7 (CD319), clone 162, Cat. No. MABF2093, is a mouse monoclonal antibody that detects SLAM family member 7 and has been tested for use in Flow Cytometry, Function Analysis, Immunohistochemistry (Paraffin), Immunoprecipitation, and Inhibition studies.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected SLAMF7 (CD319) in human bone marrow tissue sections.

Flow Cytometry Analysis: A representative lot detected SLAMF7 (CD319) in Flow Cytometry applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21).

Affects Function Analysis: A representative lot stimulated SLAMF7, which resulted in an increase SHIP-1 tyrosine phosphorylation in OPM2 and MM1S cells expressing EGFR-CD45. (Guo, H., et. al. (2015). Mol Cell Biol. 35(1):41-51).

Inhibition Analysis: A representative lot blocked the augmented capacity of human blood-derived macrophages to engulf Raji cells in response to anti-CD47 antibodies. (Chen, J., et. al. (2017). Nature. 544(7651):493-497).

Immunoprecipitation Analysis: A representative lot immunoprecipitated SLAMF7 (CD319) in Immunopreciptation applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21; Tassi, I., et. al. (2005). J Immunol. 175(12):7996-8002).
Research Category
Inflammation & Immunology

Quality

Evaluated by Immunohistochemistry (Paraffin) in human tonsil tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected SLAMF7 (CD319) in human tonsil tissue sections.

Target description

37.42 kDa calculated.

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2b in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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