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MABF2163

Sigma-Aldrich

Anti-CD33 Antibody, clone P67.6

clone P67.6, from mouse

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Synonym(s):
Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67
eCl@ss:
32160702

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

P67.6, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
inhibition assay: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... CD33(945)

Related Categories

General description

Myeloid cell surface antigen CD33 (UniProt: P20138; also known as Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67, CD33) is encoded by the CD33 (also known as SIGLEC3) gene (Gene ID: 945) in human. CD33 is a single-pass type I membrane sialoadhesion protein that is expressed by myeloid stem cells, myeloblasts, monoblasts, monocytes, macrophages, and granulocyte precursors and preferentially binds to alpha-2,6-linked sialic acid. CD33 is not expressed in erythrocytes, platelets, B-cells, T-cells, and NK cells. Hence, it can serve as a marker for myeloid cells. It is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off in the mature form. It contains an extracellular domain (aa 18-259), a transmembrane domain (aa 260-282), and a cytoplasmic domain (aa 283-364). CD33 contains two copies of a cytoplasmic motif (aa 338-343 and 356-361), which is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. In the immune response, CD33 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Human CD33 is reported to contain two tyrosine residues in its cytoplasmic domain (tyrosine 340 and 358) that when phosphorylated could function as docking sites for the phosphatases, SHP-1 and/or SHP-2, and enable CD33 to function as an inhibitory receptor.

Specificity

Clone P67.6 specifically detects human CD 33.

Immunogen

FMY9S5 cells expressing CD33.

Application

Anti-CD33, clone P67.6, Cat. No. MABF2163, is a mouse monoclonal antibody that detects CD33 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Inhibition assays.
Inhibition Assays: A representative lot of CD33 conjugated to calicheamicin inhibited inhibits the formation of colonies from AML marrow samples. (Hamann, P.R., et. al. (2002). Bioconjug Chem. 13(1):47-58).

Flow Cytometry Analysis: A representative lot detected CD33 in Flow Cytometry applications (Kussick, S.J., et. al. (2003). Arch Pathol Lab Med. 127(9):1140-7).

Immunocytochemistry Analysis: A representative lot detected CD33 in Immunocytochemistry applications (van Der Velden, V.H., et. al. (2001). Blood. 97(10):3197-204).
Research Category
Inflammation & Immunology

Quality

Evaluated by Flow Cytometry in THP-1 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CD33 in one milliion THP-1 cells.

Target description

39.82 kDa calculated.

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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