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MABS1274

Sigma-Aldrich

Anti-GPx4 Antibody, clone 1B4

clone 1B4, from rat

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Synonym(s):
Phospholipid hydroperoxide glutathione peroxidase, mitochondrial, GPx-4, GSHPx-4, mtPHGPx, PHGPx, Phospholipid hydroperoxide glutathione peroxidase, mitochondrial, snGPx, Sperm nuclei glutathione peroxidase
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1B4, monoclonal

species reactivity

mouse

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Gpx4(625249)

General description

Phospholipid hydroperoxide glutathione peroxidase, mitochondrial (EC 1.11.1.12; UniProt O70325; also known as GPx-4, GSHPx-4, mtPHGPx, PHGPx, Phospholipid hydroperoxide glutathione peroxidase mitochondrial, snGPx, Sperm nuclei glutathione peroxidase) is encoded by the Gpx4 gene (Gene ID 625249) in murine species. Gpx4 is a member of the glutathione peroxidase (GPx) family of peroxidases that protect cells against oxidative damage by reducing lipid hydroperoxides to their corresponding alcohols and by reducing free hydrogen peroxide to water. Eight glutathione peroxidase members (GPx1-8) have been identified in human. Inducible Gpx4-knockout in transgenic mice reveals the essential role of Gpx4 in maintaining proper kidney function, and Gpx4 depletion results in massive cell death of renal tubular epithelia in vivo. While the most abundant mammalian GPx, GPx1, that forms tetramers, GPx4 exists in the monomeric form. Three alternatively spliced GPx4 isoforms originate from the same Gpx4 gene, a cytosolic (also called the short form), a mitochondrial (also called the long form), and a nuclear form. The short form is expressed ubiquitously in embryonic and adult tissues in varying degrees, while the expression of the other two forms is restricted to testis where they exert significant functions during male gametogenesis.

Specificity

Reacts with the Mitochondrial (UniProt O70325-1), Cytoplasmic (UniProt O70325-2), and Nuclear (Q91XR9-1) isoforms.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminal sequence of mouse GPx-4.

Application

Anti-GPx4 Antibody, clone 1B4 is an antibody against GPx4 for use in Immunocytochemistry, Immunohistochemistry, Western Blotting.
Research Category
Signaling
Research Sub Category
Oxidative Stress
Western Blotting Analysis: A representative lot detected downregulated GPx-4 expression upon hydroxytamoxifen/Tam treatment of MERCreMER-expressing mouse embryonic fibroblasts (MEFs) PFa1(flox/flox)-MerCreMer whose last three Gpx4 exons were flanked by loxP sites (Seiler, A., et al. (2008). Cell Metab.;8(3):237-248).
Western Blotting Analysis: A representative lot detected downregulated GPx-4 expression in kidney tissue extracts from tamoxifen/Tam-fed transgenic mice (CreERT2;Gpx4fl/fl) that express Cre-ERT2 and have both Gpx4 alleles flanked by loxP sites (Friedmann Angeli, J.P., et al. (2014). Nat. Cell Biol.16(12):1180-1191).
Western Blotting Analysis: A representative lot detected downregulated GPx-4 expression upon hydroxytamoxifen/Tam treatment of lung fibroblasts derived from transgenic mice (CreERT2;Gpx4fl/fl) that express Cre-ERT2 and have both Gpx4 alleles flanked by loxP sites (Friedmann Angeli, J.P., et al. (2014). Nat. Cell Biol.16(12):1180-1191).
Western Blotting Analysis: A representative lot detected high GPx-4 expression in both primary (HRPEitC) and transformed (HK-2) human tubular epithelial kidney cells (Friedmann Angeli, J.P., et al. (2014). Nat. Cell Biol.16(12):1180-1191).
Immunohistochemistry Analysis: A representative lot detected GPx-4 expression in paraformaldehyde-fixed, paraffin-embedded mouse testis sections (Courtesy of Dr. Marcus Conrad, Helmholtz Zentrum München, Germany).
Immunohistochemistry Analysis: A representative lot detected downregulated GPx-4 expression in paraformaldehyde-fixed, paraffin-embedded testis sections from GPx-4-knockout mice when compared to wild-type controls (Schneider, M., et al. (2009). FASEB J. 23(9):3233-3242).
Immunocytochemistry Analysis: A representative lot detected downregulated GPx-4 expression in paraformaldehyde-fixed, Triton X-100-permeablized epididymal spermatozoa from Gpx4-knockout mice when compared to wild-type controls by fluorescent immunocytochemistry (Schneider, M., et al. (2009). FASEB J. 23(9):3233-3242).

Quality

Evaluated by Immunocytochemistry in MEF1 cells.

Immunocytochemistry Analysis: 4.0 µg/mL of this antibody detected GPx-4 in MEF1 cells.

Target description

~19 kDa calculated

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Jumpei Ito et al.
eLife, 10 (2021-02-03)
Heart failure is a major public health problem, and abnormal iron metabolism is common in patients with heart failure. Although iron is necessary for metabolic homeostasis, it induces a programmed necrosis. Iron release from ferritin storage is through nuclear receptor

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