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MAIPNTR10

Millipore

MultiScreen IP Filter Plate, 0.45 µm, clear, non-sterile

This clear 96 well filter plate has a Hydrophobic PVDF membrane used for PAMPA.

eCl@ss:
32039006
NACRES:
NB.22

material

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

Quality Level

sterility

non-sterile

product line

MultiScreen®

feature

hydrophobic
lid

application(s)

parallel artificial membrane permeation assay (PAMPA): suitable

plate size

96 wells

well maximum volume

300 μL

working volume

50-250 μL

matrix

Immobilon®-P

pore size

0.45 μm pore size

test parameters

sample volume: 50-250 μL (per well)

binding type

high binding surface

shipped in

ambient

Related Categories

General description

Filter Code: IP
Increasing drug candidate predictability earlier in the discovery pipeline using non-cell-based assays can improve candidate throughput during the ADME stage. Non-cell-based permeability assays are predictive models for oral absorption of development candidates.

MultiScreen Permeability, MultiScreen-IP, and MultiScreen Acceptor plates support artificial membrane strategies for predicting passive transport.

Membranes have uniform pore structure and density for reliable transport:
- Individual wells minimize candidate cross-talk
- Compatibility with automated liquid handling systems increases throughput

Permeability Assay:
- The MultiScreen Permeability plate has a polycarbonate membrane, which supports a hexane/hexadecane artificial layer for permeability assays.1

PAMPA Assay:
- The MultiScreen-IP plate has a hydrophobic PVDF membrane, which supports a lipid bilayer for PAMPA asays.2,3

MultiScreen Acceptor Plate:
- The MultiScreen Acceptor plate can be used with either plate to capture passively transported compounds. Constructed of 100% PTFE, the plate can improve LC/MS results depending on the charge of the small molecules being evaluated.

Application

Research Category
Assays

Protein

Physical form

Color Code: Clear

Other Notes

Automation Compatible: No

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificate of Analysis

Certificate of Origin

Articles

Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA)

This is a white paper on the Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA) using MultiScreen® Filter Plates.

Membrane Integrity Test for Lipid-PAMPA Artificial Membranes

This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes

Combining Permeability and Solubility Analyses in a Single Workflow

This article details the benefits of combining solubility and a non-cell based permeability assay (Parallel Artificial Membrane Permeation Assay; PAMPA) into a single workflow in which a portion of the filtrate from the MultiScreen® Solubility filter plate is added to the donor compartment of the MultiScreen filter plate for the PAMPA analysis.

Protocols

HDM-PAMPA with the MultiScreen Permeability Filter Plate

HDM-PAMPA with the MultiScreen Permeability Filter Plate

Related Content

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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