PVDF membrane (hydrophilic)
flat bottom wells
activity assay: suitable (protein kinase/phosphatase precipitation)
bead-based assay: suitable
ligand binding assay: suitable
receptor binding assay: suitable
0.22 μm pore size
sample volume: 50-250 μL (per well)
low binding surface
The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.
This web page shows how the MultiScreenHTS 384-well filter plate is used to perform receptor-ligand binding assays using the Human muscarinic M1 G-protein Coupled Receptor (GPCR) as a representative model system.
This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.