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PICM03050

Millipore

Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm

Hydrophilic PTFE cell culture insert with pore size of 0.4 µm used in a 6-well plate for Cell Attachment, Cell Culture, Cell Differentiation, Fluorescence & Low Protein Binding.

Synonym(s):
tissue culture plate, tissue culture insert, permeable culture insert, cell culture inserts
eCl@ss:
32011202
NACRES:
NB.22

Quality Level

material

PTFE membrane (hydrophilic)
flat wells
polystyrene

sterility

sterile

feature

lid: no

packaging

pack of 50

manufacturer/tradename

Millicell®

parameter

50 °C max. temp.

application(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable

H

13 mm

diam.

3.15 cm

filter diam.

30 mm

filtration area

4.2 cm2

plate size

1 wells

working volume

1.5 mL

matrix

Biopore

pore size

0.4 μm pore size

input

sample type cardiac stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)

surface area

4.2 cm2

binding type

low binding surface

detection method

fluorometric

shipped in

ambient

General description

Device Configuration: 6-well plate, 0.4μm pore size

With Millicell® inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.

Millicell® Standing Inserts:
- Promotes excellent cell growth and provides an exceptional opportunity for cell studies

Membrane Type:
Biopore Membrane (hydrophilic PTFE)
- For low protein-binding, live cell viewing, and immunofluorescent applications

Applications
Cell Attachment, Cell Growth, Cell Differentiation , Immunocytochemistry

Packaging

50 pk in Individually blister packaged
Individually blister packaged

Application

Hydrophilic PTFE cell culture insert with pore size of 0.4 µm used in a 6-well plate for Cell Attachment, Cell Culture, Cell Differentiation, Fluorescence & Low Protein Binding.
Research Category
Cell Culture

Legal Information

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificate of Analysis

Certificate of Origin

CB1 receptors and post-ischemic brain damage: Studies on the toxic and neuroprotective effects of cannabinoids in rat organotypic hippocampal slices.
Landucci, Elisa, et al.
Neuropharmacology, 60, 674-682 (2011)
Synapse elimination in olivo-cerebellar explants occurs during a critical period and leaves an indelible trace in Purkinje cells.
Letellier, Mathieu, et al.
Proceedings of the National Academy of Sciences of the USA, 106, 14102-14107 (2009)
Daniela Buonvicino et al.
British journal of pharmacology, 177(14), 3342-3356 (2020-03-22)
Drugs able to counteract progressive multiple sclerosis (MS) represent a largely unmet therapeutic need. Even though the pathogenesis of disease evolution is still obscure, accumulating evidence indicates that mitochondrial dysfunction plays a causative role in neurodegeneration and axonopathy in progressive...
Viktoria Melcher et al.
Acta neuropathologica, 139(5), 913-936 (2019-12-19)
Atypical teratoid/rhabdoid tumors (ATRT) are known for their heterogeneity concerning pathophysiology and outcome. However, predictive factors within distinct subgroups still need to be uncovered. Using multiplex immunofluorescent staining and single-cell RNA sequencing we unraveled distinct compositions of the immunological tumor...
Preparation of organotypic hippocampal slice cultures: interface method.
De Simoni, Anna and Yu, Lily M Y
Nature Protocols, 1, 1439-1445 (2006)

Protocols

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Related Content

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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