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PICM0RG50

Millipore

Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm

Hydrophilic PTFE cell culture insert with pore size of 0.4 µm used in a 6-well plate for Cell Attachment, Cell Culture, Cell Differentiation, Fluorescence & Low Protein Binding.

Synonym(s):
PICMORG50, tissue culture plate, tissue culture insert, permeable culture insert, cell culture inserts
eCl@ss:
32011202
NACRES:
NB.22

material

PTFE membrane (hydrophilic)
flat wells
polystyrene

Quality Level

sterility

sterile

feature

lid: no

packaging

pack of 48

manufacturer/tradename

Millicell®

parameter

50 °C max. temp.

application(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable

H

5 mm

diam.

3.15 cm

filter diam.

30 mm

filtration area

4.2 cm2

plate size

1 wells

matrix

Biopore

pore size

0.4 μm pore size

input

sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type: human embryonic stem cell(s)
sample type cardiac stem cell(s)

surface area

4.2 cm2

binding type

low binding surface

detection method

fluorometric

shipped in

ambient

General description

Device Configuration: 6-well plate, 0.4μm pore size

With Millicell® inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.

Millicell® Organotypic Insert:
- For high cell viability and superior study of three dimensional explant structure
- Lower height allows them to fit inside a standard petri dish
- The Biopore (PTFE) membrane provides high viability—for as long as 40 days—and excellent trans-membrane oxygen transport
- The membrane is optically clear and optimized for long-term organotypic explant maintenance

Membrane Type:
Biopore Membrane (hydrophilic PTFE)
- For low protein-binding, live cell viewing, and immunofluorescent applications

Applications
Cell Attachment, 3D Cell Culture, Cell Growth, Cell Differentiation , Immunocytochemistry

Stem Cell Types:
Mouse Embryonic Stem Cells
Human Embryonic Stem Cells
Mesenchymal Stem Cells
Neural Stem Cells
Hematopoietic Stem Cells
Epithelial Cells
Pancreatic Stem Cells
Cardiac Stem Cells
Induced Pluripotent Stem Cells

Packaging

50 pk in Individually blister packaged
Individually blister packaged

Application

Hydrophilic PTFE cell culture insert with pore size of 0.4 µm used in a 6-well plate for Cell Attachment, Cell Culture, Cell Differentiation, Fluorescence & Low Protein Binding.
Research Category
Cell Culture

Legal Information

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10-13 - German Storage Class 10 to 13

Certificate of Analysis

Certificate of Origin

Keisuke Ikegami et al.
Investigative ophthalmology & visual science, 61(3), 26-26 (2020-03-18)
Elevated IOP can cause the development of glaucoma. The circadian rhythm of IOP depends on the dynamics of the aqueous humor and is synchronized with the circadian rhythm pacemaker, that is, the suprachiasmatic nucleus. The suprachiasmatic nucleus resets peripheral clocks...
Ryota Tamura et al.
Human gene therapy, 31(5-6), 352-366 (2020-02-23)
Glioblastoma is the most aggressive brain tumor characterized by diffuse infiltration into the normal brain parenchyma. Neural stem cells are known to possess the tumor-tropic migratory capacity and thus can be used as cellular vehicles for targeted delivery of therapeutic...
Abigail K Myers et al.
Development (Cambridge, England), 147(2) (2020-01-10)
The precise migration of cortical interneurons is essential for the formation and function of cortical circuits, and disruptions to this key developmental process are implicated in the etiology of complex neurodevelopmental disorders, including schizophrenia, autism and epilepsy. We have recently...
P Hardman et al.
Development, growth & differentiation, 35(6), 683-690 (1993-01-01)
Embryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane...
Andrea S Cupp et al.
Biology of reproduction, 68(6), 2033-2037 (2003-02-28)
The first morphological event after initiation of male sex determination is seminiferous cord formation in the embryonic testis. Cord formation requires migration of pre-peritubular myoid cells from the adjacent mesonephros. The embryonic Sertoli cells are the first testicular cells to...

Protocols

Toluidine Blue Staining Protocol for Millicell® Culture Inserts

This is a Toluidine Blue Staining protocol listing materials and methods.

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Related Content

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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