Vacuum filter cups are commonly used in the industry to degas solutions. For some liquid chromatography systems, applying a vacuum while filtering the mobile phase may remove enough gas to prevent issues.
About This Item
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material
polyethersulfone membrane
polystyrene funnel(s)
polystyrene housing
Quality Level
sterility
sterile; irradiated
product line
Steritop®
feature
hydrophilic
packaging
pack of 12 ea
parameter
45 °C max. temp.
description
2.4 mL (hold-up volume)
technique(s)
sterile filtration: suitable
H
95 mm
diam.
12.5 cm
filter capacity
250 mL
filter diam.
73 mm
filtration area
40 cm2
matrix
Express PLUS™
pore size
0.22 μm pore size
fitting
inlet funnel
45 mm outlet thread(s)
shipped in
ambient
1 of 4
This Item | S2GPT10RE | S2GPT01RE | SCGPS01RE |
|---|---|---|---|
| sterility sterile; irradiated | sterility sterile; irradiated | sterility sterile; irradiated | sterility sterile; irradiated |
| material polyethersulfone membrane, polystyrene housing, polystyrene funnel(s) | material polyethersulfone membrane, polystyrene funnel(s), polystyrene housing | material polyethersulfone membrane, polystyrene funnel(s), polystyrene housing | material polyethersulfone membrane, polystyrene funnel(s), polystyrene housing |
| pore size 0.22 μm pore size | pore size 0.22 μm pore size | pore size 0.22 μm pore size | pore size 0.22 μm pore size |
| packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea |
| feature hydrophilic | feature hydrophilic | feature hydrophilic | feature hydrophilic |
| fitting inlet funnel | fitting inlet funnel, 45 mm outlet thread(s) | fitting inlet funnel, 45 mm outlet thread(s) | fitting inlet funnel, 33 mm outlet thread(s) |
General description
Stericup® and Steritop® sterile filtration devices use reliable, high-performance membranes for optimum sterile filtration of laboratory reagents, buffers, and media. These devices combine superior flow rates and throughput with low non-specific binding and a stable, no-tip design.
This Steritop® 250 mL bottle-top with 0.22 μm pore size polyethersulfone (PES) membrane filter unit can be used on bottles with 45 mm openings, designed for vacuum applications.
Application
- Tissue culture media +/- additives
- Buffers
- Biological solutions
Features and Benefits
Legal Information
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Evaluation of sterile filtration devices for protein preservation in sterile filtration of cell culture media.
Select the right sterile filter by media, volume, and application.
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This article shows that a PES membrane containing Stericup Quick Release filter provides an alternative for the pluripotent expansion of embryonic stem cells.
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Can you use filtration units to filter and also degas solutions?
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What is the maximum processing volume for Stericup® and Steritop® filters? Can I add more volume after initial filtration?
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All Stericup® and Steritop® filters were developed for single-use application. The maximum volume is dependent on the receiver bottle capacity. Stericup® filters come with a receiver bottle with a specified maximum volume. For Steritop® filters, the maximum recommended volume is 2 L with an appropriate-sized receiver bottle. Larger processing volumes can result in membrane fouling, increase chance of spillage and contamination, and lead to possible airlock*.
*Note that devices with PES membranes can airlock if filtered to dryness. Some liquid must be left on top of the filter, then the system stopped, receiver changed or emptied, and more sample volume added before restart. This increases the chance of spillage.Helpful?
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What is the maximum vacuum pressure for filtration?
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700 mm Hg (27.5” Hg, 0.9 bar) differential vacuum at 25 °C (77 °F)
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Are Stericup® and Steritop® filters GMP-certified?
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No, Stericup® and Steritop® devices are not GMP-approved.
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Can Stericup® and Steritop® filters be reused?
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All Stericup® and Steritop® filters were developed for single use, disposable processing.
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Can Stericup® and Steritop® filters be used to prepare solutions that will be administered to patients?
1 answer-
No, all Stericup® and Steritop® devices are Research Use Only.
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What Bacterial Endotoxins Test is used?
1 answer-
The test method used complies with USP 85 and EP 2.6.14 to ensure the absence of endotoxin in our products.
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Which type of pump should be used with Stericup® and Steritop® vacuum filters?
Which type of pump should be used with Stericup® and Steritop® vacuum filters?
1 answer-
We recommend our Chemical Duty Pump: https://www.sigmaaldrich.com/product/mm/wp6111560
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What leachables are detected during filtration processes using Stericup® filters?
1 answer-
We have not performed extractables/leachables studies for our Stericup® filters.
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What is the chemical compatibility of the various Stericup® and Steritop® filters?
What is the chemical compatibility of the various Stericup® and Steritop® filters?
1 answer-
Please refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf
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