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S2GPT02RE

Millipore® Steritop® Vacuum Bottle Top Filter

pore size 0.22 μm, polyethersulfone membrane, filter capacity 250 mL, pack of 12 ea

Synonym(s):

SCGPT02RE

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About This Item

UNSPSC Code:
41104914
NACRES:
NB.24
eCl@ss:
32031623
Pore size:
0.22 μm pore size
Filter capacity:
250 mL
Packaging:
pack of 12 ea
Material:
polyethersulfone membrane, polystyrene funnel(s), polystyrene housing
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Quality Segment

material

polyethersulfone membrane, polystyrene funnel(s), polystyrene housing

product line

Steritop®

feature

hydrophilic

packaging

pack of 12 ea

parameter

45 °C max. temp.

description

2.4 mL (hold-up volume)

technique(s)

sterile filtration: suitable

H

95 mm

diam.

12.5 cm

filter capacity

250 mL

filter diam.

73 mm

filtration area

40 cm2

matrix

Express PLUS™

pore size

0.22 μm pore size

fitting

inlet funnel, 45 mm outlet thread(s)

shipped in

ambient

storage temp.

no temp limit

General description

This item is a direct replacement for SCGPT02RE
Work with ease and filter with confidence with Stericup® and Steritop® Filtration Systems.

Stericup® and Steritop® sterile filtration devices use reliable, high-performance membranes for optimum sterile filtration of laboratory reagents, buffers, and media. These devices combine superior flow rates and throughput with low non-specific binding and a stable, no-tip design.

This Steritop® 250 mL bottle-top with 0.22 μm pore size polyethersulfone (PES) membrane filter unit can be used on bottles with 45 mm openings, designed for vacuum applications.

Application

For Research Use Only
Stericup® and Steritop® systems are designed to reduce foaming and protein denaturation while maximizing flow and can be used for sterile vacuum filtration of aqueous solutions such as tissue culture media and biological fluids.

  • Tissue culture media +/- additives
  • Buffers
  • Biological solutions

Features and Benefits

Bold product identification on the Steritop® filter funnel plainly displays membrane characteristics, including composition and pore size, for rapid, application-appropriate product selection.

Legal Information

EXPRESS PLUS is a trademark of Merck KGaA, Darmstadt, Germany
Millipore is a registered trademark of Merck KGaA, Darmstadt, Germany
STERITOP is a registered trademark of Merck KGaA, Darmstadt, Germany

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S2GPT10RES2GPT05RES2GPT01RE
description

pore size 0.22 μm, polyethersulfone membrane, filter capacity 250 mL, pack of 12 ea

description

pore size 0.22 μm, polyethersulfone membrane, filter capacity 1000 mL, pack of 12 ea

description

pore size 0.22 μm, polyethersulfone membrane, filter capacity 500 mL, pack of 12 ea

description

pore size 0.22 μm, polyethersulfone membrane, filter capacity 150 mL, pack of 12 ea

pore size

0.22 μm pore size

pore size

0.22 μm pore size

pore size

0.22 μm pore size

pore size

0.22 μm pore size

filter capacity

250 mL

filter capacity

1000 mL

filter capacity

500 mL

filter capacity

150 mL

material

polyethersulfone membrane, polystyrene housing, polystyrene funnel(s)

material

polyethersulfone membrane, polystyrene housing, polystyrene funnel(s)

material

polystyrene funnel(s), polystyrene housing, polyethersulfone membrane

material

polyethersulfone membrane, polystyrene housing, polystyrene funnel(s)

fitting

inlet funnel, 45 mm outlet thread(s)

fitting

inlet funnel, 45 mm outlet thread(s)

fitting

45 mm outlet thread(s), inlet funnel

fitting

45 mm outlet thread(s), inlet funnel

packaging

pack of 12 ea

packaging

pack of 12 ea

packaging

pack of 12 ea

packaging

pack of 12 ea

feature

hydrophilic

feature

hydrophilic

feature

hydrophilic

feature

hydrophilic


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Questions

1–10 of 17 Questions  
  1. Can you use filtration units to filter and also degas solutions?

    1 answer
    1. Vacuum filter cups are commonly used in the industry to degas solutions. For some liquid chromatography systems, applying a vacuum while filtering the mobile phase may remove enough gas to prevent issues.

      Helpful?

  2. What is the difference between classic Stericup® filters and Stericup® “Quick Release” filters?

    1 answer
    1. Stericup® Quick Release, or “QR”, filters feature a connection between funnel filter and receiver bottle that requires only a quarter-turn release to facilitate funnel removal. These filters come fully engaged – there is no need to tighten before use. The receiver bottle cap has flanges on the top surface to improve grip, as well as a positive lock stop to ensure a leak-proof seal with the receiver bottle. Other improvements include a large, frosted writing surface on the receiver bottle and a lighter cap color to facilitate labelling, more prominent volume markings on the filtration funnel and receiver bottle, and a product label with a peel-off lot record strip on the bag for easy viewing in storage.

      Helpful?

  3. What is the expiration date of Stericup® and Steritop® filters?

    1 answer
    1. Typically 3 years from sterilization.

      Helpful?

  4. What tubing can be used with Stericup® and Steritop® vacuum filters?

    What tubing can be used with Stericup® and Steritop® vacuum filters?

    1 answer
    1. Stericup® and Steritop® filters are fitted with a 1/4” tube adapter. We recommend 3/16 in. (4.8 mm) ID silicone tubing to fit this tube adapter: https://www.sigmaaldrich.com/product/mm/xx7100004

      Helpful?

  5. What is the maximum vacuum pressure for filtration?

    1 answer
    1. 700 mm Hg (27.5” Hg, 0.9 bar) differential vacuum at 25 °C (77 °F)

      Helpful?

  6. What is the chemical compatibility of the various Stericup® and Steritop® filters?

    What is the chemical compatibility of the various Stericup® and Steritop® filters?

    1 answer
    1. Helpful?

  7. The User Guide says “Use only glass or plastic bottles designed for vacuum applications”. Can you specify which bottles are compatible?

    1 answer
    1. Steritop® Quick Release bottle top units require a vacuum-rated receiver bottle with either a GL33 or GL45 mm closure thread as specified. Steritop® E bottle top units require a vacuum-rated receiver bottle with a GL45 mm closure thread. Bottles no larger than 2 L should be used.
      Glass bottles, GL33:
      • Wheaton media bottles (glass), 500 mL (Product Z364886)
      • Wheaton media bottles (glass), 1 L (Product Z364894)
      Glass bottles, GL45
      • Corning® glass media storage bottle, 125 mL (Product CLS1399125)
      • Corning® glass media storage bottle, 250 mL (Product CLS1399250)
      • Corning® glass media storage bottle, 500 mL (Product CLS1399500)
      • Corning® glass media storage bottle, 1 L (Product CLS13991L)
      • Pyrex® round media storage bottle, 500 mL (Product CLS1395500)
      • Pyrex® round media storage bottle, 1 L (Product CLS13951L)
      • KIMBLE® KIMAX® Media bottle, 250 mL (Product DWK14395250)
      • KIMBLE® KIMAX® Media bottle, 500 mL (Product DWK14395500)
      • KIMBLE® KIMAX® Media bottle, 1 L (Product DWK143951000)
      Plastic (pre-sterile) bottles, GL45:
      • Stericup® Quick Release Receiver flask, 250 mL (Product S200B02RE)
      • Stericup® Quick Release Receiver flask, 500 mL (Product S200B05RE)
      • Stericup® Quick Release Receiver flask, 1000 mL (Product S200B10RE)

      Helpful?

  8. Can we filter oil-type liquids with Stericup® filters?

    Can we filter oil-type liquids with Stericup® filters?

    1 answer
    1. Likely not, due to pore size/possible fouling/chemical compatibility. Refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf

      Helpful?

  9. What leachables are detected during filtration processes using Stericup® filters?

    1 answer
    1. We have not performed extractables/leachables studies for our Stericup® filters.

      Helpful?

  10. Can Stericup® bottles be placed inside freezers for long-term storage of media?

    1 answer
    1. You can successfully freeze and store many aqueous solutions (such as culture media) in Stericup® bottles at temperatures up to -20 °C (-4 °F). It is strongly recommended that you run a sample stability trial under your actual storage conditions prior to using Stericup® bottles for frozen storage.

      Helpful?

1–10 of 17 Questions  

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