The Stericup products are not designed for their filter to be removed. Users can choose to cut membranes out of the product. However, there is no designed way to perform this.
About This Item
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material
PVDF membrane
polystyrene bottle
polystyrene funnel(s)
polystyrene housing
Quality Level
sterility
sterile; irradiated
product line
Stericup®
feature
hydrophilic
packaging
pack of 12 ea
parameter
45 °C max. temp.
description
2.4 mL (hold-up volume)
technique(s)
sterile filtration: suitable
H
263 mm
diam.
12.5 cm
filter capacity
500 mL
filter diam.
73 mm
filtration area
40 cm2
matrix
Durapore®
pore size
0.45 μm pore size
fitting
inlet funnel
outlet flask (Receiver)
shipped in
ambient
1 of 4
This Item | S2HVU02RE | S2HVU01RE | S2GVU10RE |
|---|---|---|---|
| pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.22 μm pore size |
| sterility sterile; irradiated | sterility sterile; irradiated | sterility sterile; irradiated | sterility sterile; irradiated |
| material PVDF membrane, polystyrene funnel(s), polystyrene bottle, polystyrene housing | material PVDF membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing | material PVDF membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing | material PVDF membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing |
| packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea |
| fitting inlet funnel, outlet flask (Receiver) | fitting inlet funnel, outlet flask (Receiver) | fitting inlet funnel, outlet flask (Receiver) | fitting inlet funnel, outlet flask (Receiver) |
| feature hydrophilic | feature hydrophilic | feature hydrophilic | feature hydrophilic |
General description
Stericup® and Steritop® vacuum sterile filtration devices use reliable, high-performance membranes for optimum sterile filtration of laboratory reagents, buffers, and media. These devices combine superior flow rates and throughput with low non-specific binding and a stable, no-tip design.
This Stericup® 0.45 μm pore size polyvinylidene fluoride (PVDF) membrane filter unit combines a 500 mL bottle-top with a 500 mL receiver flask and cap for processing and storage.
Application
- Tissue culture media +/- additives
- Buffers
- Biological solutions
Features and Benefits
- The Quick Release filter funnel disconnects from the receiver bottle with just a quarter turn—reducing the likelihood of spillage and making it easier to manipulate on the bench or under the hood.
- Bold product identification on the Steritop® filter funnel plainly displays membrane characteristics, including composition and pore size, for rapid, application-appropriate product selection.
- Click seal cap is flanged and modified to enable a firm grip in wet or dry conditions—and a tactile stop confirms secure closure. Worry less about the possibility of spilling or contamination of sterilized contents.
- Frosted, ample writing surface on the receiver bottle and lighter cap color facilitate clear labelling to improve legibility and reduce unreadable smears.
- Click seal cap rests on the side to avoid risk of contamination.
- Receiver bottles are stackable to save storage space.
Legal Information
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Certificates of Analysis (COA)
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Articles
Select the right sterile filter by media, volume, and application.
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I want to pull sample through the filter and then perform a nucleic acid extraction on the filter. Is this possible? Can the filter be easily removed?
1 answer-
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What is the maximum processing volume for Stericup® and Steritop® filters? Can I add more volume after initial filtration?
1 answer-
All Stericup® and Steritop® filters were developed for single-use application. The maximum volume is dependent on the receiver bottle capacity. Stericup® filters come with a receiver bottle with a specified maximum volume. For Steritop® filters, the maximum recommended volume is 2 L with an appropriate-sized receiver bottle. Larger processing volumes can result in membrane fouling, increase chance of spillage and contamination, and lead to possible airlock*.
*Note that devices with PES membranes can airlock if filtered to dryness. Some liquid must be left on top of the filter, then the system stopped, receiver changed or emptied, and more sample volume added before restart. This increases the chance of spillage.Helpful?
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What is the maximum vacuum pressure for filtration?
1 answer-
700 mm Hg (27.5” Hg, 0.9 bar) differential vacuum at 25 °C (77 °F)
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Can Stericup® and Steritop® filters be used to prepare solutions that will be administered to patients?
1 answer-
No, all Stericup® and Steritop® devices are Research Use Only.
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Can Stericup® and Steritop® filters be reused?
1 answer-
All Stericup® and Steritop® filters were developed for single use, disposable processing.
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What is the expiration date of Stericup® and Steritop® filters?
1 answer-
Typically 3 years from sterilization.
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Can Stericup® bottles be placed inside freezers for long-term storage of media?
1 answer-
You can successfully freeze and store many aqueous solutions (such as culture media) in Stericup® bottles at temperatures up to -20 °C (-4 °F). It is strongly recommended that you run a sample stability trial under your actual storage conditions prior to using Stericup® bottles for frozen storage.
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Which type of pump should be used with Stericup® and Steritop® vacuum filters?
Which type of pump should be used with Stericup® and Steritop® vacuum filters?
1 answer-
We recommend our Chemical Duty Pump: https://www.sigmaaldrich.com/product/mm/wp6111560
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What Bacterial Endotoxins Test is used?
1 answer-
The test method used complies with USP 85 and EP 2.6.14 to ensure the absence of endotoxin in our products.
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Can we filter oil-type liquids with Stericup® filters?
Can we filter oil-type liquids with Stericup® filters?
1 answer-
Likely not, due to pore size/possible fouling/chemical compatibility. Refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf
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