114367

Bromocresol Green sodium salt

ACS reagent, Dye content 90 %

• Synonym(s): 3′,3″,5′,5″-Tetrabromo-m-cresolsulfonphthalein sodium salt
• Empirical Formula (Hill Notation): C₂₁H₁₃Br₄NaO₅S
• CAS Number: 62625-32-5
• Molecular Weight: 720.00
• EC Number: 263-657-4
• MDL number: MFCD00148898
• PubChem Substance ID: 24847167

Properties

• grade: ACS reagent
• Quality Level: 200
• assay: 90% (HPLC)
• form: powder or crystals
• composition: Dye content, 90%
• visual transition interval: 3.8-5.2, yellow to blue
• mp: 230 °C (dec.) (lit.)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].Cc1c(Br)c(O)c(Br)cc1\C(=C2\C=C(Br)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O
• InChI: 1S/C21H14Br4O5S.Na/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21;/h3-8,26-27H,1-2H3;/q;+1/p-1
• InChI key: HEFSGAHJDGZCHA-UHFFFAOYSA-M

Description

General description

Bromocresol Green (BCG), also known as Bromcresol Green, is an acidic dye and belongs to the phthalein and sulphonphthalein dyes group. The dye color changes from yellow to blue-green at pH 3.8-5.4. The monosodium salt of the dye is 4% soluble in water and 6% in ethanol. The dye at a higher pH level produces a large and weakly lipophilic dianion.

Application

Bromocresol Green (BCG) is used widely as a vital stain to study blood-brain permeability. Mainly, BCG serves as a pH indicator in biology and medicinal experiments. Bromocresol Green is suitable as a selective inhibitor in kidney physiology studies. BCG is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes. In TLC BCG is used for the visualisation of the compounds with functional groups whose pKa is below 5.0.

Packaging

1, 5, 25 g in glass bottle

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves