This Calcofluor White solution is prepared in water and packaged in an amber glass bottle. Provided the solution is not contaminated or allowed to evaporate, the product is expected to remain stable at least until the assigned retest date published in the lot-specific Certificate of Analysis.
https://www.sigmaaldrich.com/product/sial/18909#product-documentation
18909
Calcofluor White Stain
suitable for microbiology
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SMILES string
C1=CC=C(C=C1)NC2=NC(=NC(=N2)N(CCO)CCO)NC3=CC(=C(C=C3)C=CC4=C(C=C(C=C4)NC5=NC(=NC(=N5)NC6=CC=CC=C6)N(CCO)CCO)S(=O)(=O)O)S(=O)(=O)O
InChI
InChI=1S/C40H44N12O10S2/c53-21-17-51(18-22-54)39-47-35(41-29-7-3-1-4-8-29)45-37(49-39)43-31-15-13-27(33(25-31)63(57,58)59)11-12-28-14-16-32(26-34(28)64(60,61)62)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52(19-23-55)20-24-56/h1-16,25-26,53-56H,17-24H2,(H,57,58,59)(H,60,61,62)(H2,41,43,45,47,49)(H2,42,44,46,48,50)/b12-11+
InChI key
CNGYZEMWVAWWOB-VAWYXSNFSA-N
product line
BioChemika
form
liquid
composition
Calcofluor White M2R, 1 g/L , Evans blue, 0.5 g/L
technique(s)
microbe id | staining: suitable
color
blue to very dark blue
λmax
423-443 nm
fluorescence
λex 423-443 nm in 0.1 M phosphate pH 7.0
application(s)
food and beverages
hematology
histology, microbiology
storage temp.
room temp
suitability
fungi, parasites, yeasts
Quality Level
1 of 4
This Item | |||
|---|---|---|---|
| suitability fungi, yeasts, parasites | suitability Bacillus spp., Corynebacterium spp. | suitability Chlamydia spp., Histoplasma spp., Plasmodium spp., Trichomonas spp., bacteria, protozoa, spirochetes | suitability bacteria |
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable |
| color blue to very dark blue | color - | color - | color - |
| fluorescence λex 423-443 nm in 0.1 M phosphate pH 7.0 | fluorescence - | fluorescence - | fluorescence - |
| application(s) food and beverages | application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing |
| form liquid | form liquid | form - | form liquid |
General description
Calcofluor White Stain (C.l. 40622) is a stilbene compound that has additional triazinyl and phenyl aromatic groups. It is also known as cellufluor.
Application
Calcofluor White Stain is a fluorescent stain that is used for the rapid detection of yeasts, fungi, and parasitic organisms. It is a non-specific fluorochrome that binds to cellulose and chitin in cell walls. It is suitable for use as a viability stain for plant cells. Additionally, calcofluor white is recommended as a counterstain for in situ hybridization staining of plant material and as a flow cytometry stain for assessing the viability of animal cells.
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Eye Irrit. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Weiwei Dang et al.
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Hokyoung Son et al.
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Fusarium graminearum (teleomorph Gibberella zeae) is a prominent pathogen that infects major cereal crops such as wheat, barley, and maize. Both sexual (ascospores) and asexual (conidia) spores are produced in F. graminearum. Since conidia are responsible for secondary infection in
Melphine M Harriott et al.
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Related Content
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Can you tell us about the stability of the product once the bottle is opened?
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Is this an aqueous solution? what is the solvent? I haven't been able to find that information.
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Yes, this product is an aqueous solution containing 1 g/L Calcofluor White and 0.5 g/L Evans Blue. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/177/132/18909bul-ms.pdfHelpful?
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