Merck
All Photos(2)

53286

Millipore

Brain Heart Infusion Broth

suitable for microbiology, NutriSelect® Plus

Synonym(s):
BHI Broth, Brain Heart Broth
NACRES:
NA.74

Agency

according to ISO 6888-1 2020
according to ISO 6888-3:2003

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

beef heart (infusion from 250g), 5 g/L
calf brains (infusion from 200g), 12.5 g/L
disodium hydrogen phosphate, 2.5 g/L
D(+)-glucose, 2 g/L
peptone, 10 g/L
sodium chloride, 5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
life science and biopharma
pathogen testing
pharmaceutical

microbiology

suitability

Haemophilus spp.
nonselective for Bacteroides spp.
nonselective for Candida spp.
nonselective for Escherichia coli
nonselective for Neisseria spp.
nonselective for Pseudomonas spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for Yersinia spp.
nonselective for bacteria (General Media)
nonselective for coliforms

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Application

Brain Heart Infusion Broth is recommended for the cultivation of fastidious pathogenic microorganisms. Brain Heart Infusion Broth has been used as culture medium forStreptococcus pneumonia, Listeria monocytogenes, Leishmania promastigotes and Clostridium difficile.

Preparation Note

Dissolve 37 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Production of 12α-hydroxysteroid dehydrogenase from Clostridium group P

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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