88597

Hydrogen peroxide solution

3%, suitable for microbiology

• Synonym(s): Catalase Test, Catalase Test, H₂O₂
• Empirical Formula (Hill Notation): H₂O₂
• CAS Number: 7722-84-1
• Molecular Weight: 34.01
• UNSPSC Code: 41171621
• NACRES: NA.85
• PubChem Substance ID: 329769664
• MDL number: MFCD00011333
• Beilstein/REAXYS Number: 3587191
• Assay: 2.8-3.2% (redox titration)
• Form: solution

Properties

• InChI: 1S/H2O2/c1-2/h1-2H
• SMILES string: OO
• InChI key: MHAJPDPJQMAIIY-UHFFFAOYSA-N
• agency: according to GB 4789.30-2016, according to ISO 10272-1:2017, according to ISO 10272-2:2017, according to ISO 11290-1:2017, according to ISO 11290-2:2017
• vapor pressure: 23.3 mmHg ( 30 °C)
• assay: 2.8-3.2% (redox titration)
• form: solution
• shelf life: limited shelf life, expiry date on the label
• storage condition: protect from light
• technique(s): microbe id | specific enzyme detection: suitable
• color: colorless
• pH: 6-8 (25 °C)
• mp: 0.0 °C
• density: 1.000 g/cm³
• application(s): clinical testing; environmental; food and beverages; veterinary, microbiology
• suitability: Pneumococcus spp., Staphylococcus spp., bacteria
• Quality Level: 200

Description

General description

Hydrogen peroxide is often used in microbiology for its ability to break down into water and oxygen gas. This reaction is catalyzed by the enzyme catalase, which is present in many aerobic and facultatively anaerobic bacteria. A small amount of hydrogen peroxide is added to a bacterial culture to detect the presence or absence of catalase activity in the culture.If catalase is present, it will catalyze the breakdown of the hydrogen peroxide, resulting in the formation of oxygen gas bubbles. This reaction can be visually observed as effervescence in the culture. The absence of bubbles indicates that catalase is not present in the culture.The catalase test is commonly used in microbial identification and differentiation, as different species of bacteria present varying levels of catalase activity. For example, Staphylococcus species exhibit high levels of catalase activity, while Streptococcus species do not. The catalase test is also used to differentiate between aerobic bacteria that produce oxygen as a by-product (which typically exhibit catalase activity) and facultative anaerobic bacteria that utilize oxygen but do not produce it as a by-product (which may or may not exhibit catalase activity).

Application

Hydrogen peroxide has several important microbial applications due to its strong oxidizing properties. For example:

• Disinfectant: Hydrogen peroxide can be used as a disinfectant for surfaces, materials, and equipment in microbiology labs. When applied to surfaces, hydrogen peroxide reacts with organic materials, such as bacteria and fungi, and destroys them through oxidation.
• Sterilization: Hydrogen peroxide is often used in sterilization procedures in microbiology, especially in the pharmaceutical and medical industries. It effectively kills spores that are resistant to many other types of disinfectants.
• Microbial detection: Hydrogen peroxide can be used to detect catalase activity in bacteria, making it useful in microbial identification and differentiation. The species of bacteria may be distinguished based on their ability to catalyze the breakdown of hydrogen peroxide.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Faceshields, Gloves, Goggles