Merck
All Photos(3)

E4884

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

ACS reagent, 99.0-101.0%

Synonym(s):
Edetate disodium salt dihydrate, Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, Edathamil, EDTA-Na2, Sequestrene Na2
Empirical Formula (Hill Notation):
C10H14N2Na2O8 · 2H2O
CAS Number:
Molecular Weight:
372.24
Beilstein:
3900609
EC Number:
PubChem Substance ID:
NACRES:
NA.77

Quality Level

grade

ACS reagent

assay

99.0-101.0%

form

solid

impurities

≤0.005% Insoluble matter
≤0.1% Nitrilotriacetic acid ([HOCOCH2]3N)

pH

4.0-6.0 (25 °C, 5%)

mp

248 °C (dec.) (lit.)

solubility

H2O: 100 g/L at 20 °C

cation traces

Fe: ≤0.01%
heavy metals (as Pb): ≤0.005%

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

General description

EDTA is a chelator of divalent cations. It inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Application

Ethylenediaminetetraacetic acid disodium salt dehydrate has been used as a component:
  • of radioimmunoprecipitation assay buffer (RIPA buffer) for preparation of C2C12 mouse skeletal myoblasts whole cell lysate
  • of lysis buffer and IgG resin wash and equilibration buffer for the purification of human proteasome regulatory particles (RPs)
  • of M-buffer for the isolation of human cardiomyocyte progenitor cells (CMPCs) from fetal heart

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Packaging

100, 500 g in poly bottle

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, majorly used in preventing clotting of blood. EDTA may trigger aggregation of platelets resulting in pseudothrombocytopenia. EDTA along with tetracycline serves as a root conditioning agent and provides relief in gum infection. EDTA-tetracycline therapy decreases coronary artery calcium levels in atherosclerosis. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It also enhances the antimicrobial functionality of lysozyme and improves shelf life of cheese.

Pictograms

Exclamation markHealth hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Inhalation - Aquatic Chronic 3 - STOT RE 2 Inhalation

Target Organs

Respiratory Tract

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Mitophagy is required for mitochondrial biogenesis and myogenic differentiation of C2C12 myoblasts
Sin J, et al.
Autophagy, 12(2), 369-380 (2016)
Conformational landscape of the p28-bound human proteasome regulatory particle
Lu Y, et al.
Molecular Cell, 67(2), 322-333 (2017)
Human Cardiomyocyte Progenitor Cells in Co-culture with Rat Cardiomyocytes Form a Pro-arrhythmic Substrate: Evidence for Two Different Arrhythmogenic Mechanisms
Smit NW, et al.
Frontiers in Physiology, 8, 797-797 (2017)
Lysozyme/EDTA disodium salt and modified-atmosphere packaging to prolong the shelf life of burrata cheese
Conte A, et al.
Journal of Dairy Science, 94(11), 5289-5297 (2011)
Serena Duchi et al.
Materials (Basel, Switzerland), 12(4) (2019-02-23)
Cartilage defects and diseases remain major clinical issues in orthopaedics. Biomanufacturing is now a tangible option for the delivery of bioscaffolds capable of regenerating the deficient cartilage tissue. However, several limitations of in vitro and experimental animal models pose serious

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service