All Photos(6)



Ethylenediaminetetraacetic acid disodium salt dihydrate

reagent grade, 98.5-101.5% (titration)

Edetate disodium salt dihydrate, Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, Edathamil, EDTA-Na2, Sequestrene Na2
Empirical Formula (Hill Notation):
C10H14N2Na2O8 · 2H2O
CAS Number:
Molecular Weight:
EC Number:
PubChem Substance ID:

Quality Level

biological source

synthetic (organic)


reagent grade


98.5-101.5% (titration)




248 °C (dec.) (lit.)


H2O: soluble, clear, colorless

storage temp.

room temp

SMILES string




InChI key


Looking for similar products? Visit Product Comparison Guide

General description

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid disodium salt (EDTA) is a blood anticoagulant and contributes to the pathogenesis of pseudothrombocytopenia. It chelates with calcium in the blood and inhibits clotting and is routinely used in haematological tests. It enhances the antibacterial activity of lysozyme. EDTA used in chelation therapy chelates with calcium and favors dilation of artery, solubilisation of atheromatous plaques in atherosclerotic vascular disease. EDTA chelation therapy protects from lipid peroxidation events in liver fibrosis.


Ethylenediaminetetraacetic acid disodium salt dihydrate has been used as a component of the plasma membrane resuspension buffer, inhibitor′s preservative solution (IS) and luciferase activity assay mix.
Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.


50, 100, 250, 500 g in poly bottle
1 kg in poly bottle
2.5, 5 kg in poly drum

Biochem/physiol Actions

Zinc-dependent metalloproteinases, as well as other proteases that are stabilized by calcium, can be effectively inhibited by chelation of divalent metal ions with EDTA. Other chelators such as EGTA, specific for calcium, and 1,10-phenanthroline, highly specific for zinc, can be used to target these two different types of proteases.


Exclamation markHealth hazard

Signal Word


Hazard Statements

Hazard Classifications

Acute Tox. 4 Inhalation - Aquatic Chronic 3 - STOT RE 2 Inhalation

Target Organs

Respiratory Tract

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Isolation of plasma membrane-associated membranes from rat liver
Suski JM, et al.
Nature Protocols, 9(2), 312-312 (2014)
Measurement of free IGF-I saliva levels: perspectives in the detection of GH/IGF axis in athletes
Antonelli G, et al.
Clinical Biochemistry, 40(8), 545-550 (2007)
Conazole fungicides inhibit Leydig cell testosterone secretion and androgen receptor activation in vitro.
Roelofs MJE, et al.
Toxicology Reports, 1, 271-283 (2014)
EDTA chelation therapy for cardiovascular disease: a systematic review
Seely DMR, et al.
BMC Cardiovascular Disorders, 5(1), 32-32 (2005)
Lysozyme/EDTA disodium salt and modified-atmosphere packaging to prolong the shelf life of burrata cheese
Conte A, et al.
Journal of Dairy Science, 94(11), 5289-5297 (2011)


Enzymatic Assay and ATP Sensitivity Test of Luciferase

Objective: To standardize a procedure to determine the enzymatic activity of Luciferase and/or to determine the ATP detection limit of Luciferase.

Enzymatic Assay of Choloylglycine Hydrolase

To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.

Enzymatic Assay of Superoxide Dismutase

Enzymatic Assay of Superoxide Dismutase

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service