A3678

Ammonium persulfate

for molecular biology, suitable for electrophoresis, ≥98%

• Synonym(s): AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER
• Linear Formula: (NH₄)₂S₂O₈
• CAS Number: 7727-54-0
• Molecular Weight: 228.20
• EC Number: 231-786-5
• MDL number: MFCD00003390
• eCl@ss: 38030421
• PubChem Substance ID: 24890800
• NACRES: NB.22

Properties

• grade: for molecular biology
• Quality Level: 200
• vapor density: 7.9 (vs air)
• Assay: ≥98%
• form: powder
• reaction suitability: reagent type: oxidant
• technique(s): electrophoresis: suitable
• anion traces: chloride (Cl^-): <10 ppm
• cation traces: Fe: <10 ppm; heavy metals (as Pb): <50 ppm
• foreign activity: DNAse, none detected; Protease, none detected; RNAse, none detected
• SMILES string: N.N.OS(=O)(=O)OOS(O)(=O)=O
• InChI: 1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)
• InChI key: ROOXNKNUYICQNP-UHFFFAOYSA-N

Description

General description

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.

Application

Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.

Catalyst for acrylamide gel polymerization.

Analysis Note

Tested for use in acrylamide polymerization.

Safety Information

• Pictograms: GHS03,GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H272 - H302 - H315 - H317 - H319 - H334 - H335
• Precautionary Statements: P210 - P220 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Ox. Sol. 3 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 5.1B - Oxidizing hazardous materials
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves