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CryoStor® cell cryopreservation media



Quality Level






cell culture | mammalian: suitable
cryopreservation: suitable

shipped in


storage temp.


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General description

The CryoStor CS2, CS5, and CS10 family of preservation solutions represents the next generation of cryopreservation media. Designed to prepare and preserve cells in ultra low temperature environments (–80 to –196 °C), CryoStor media provide a safe, protective environment for cells and tissues during thefreezing, storage, and thawing process. Through modulating the cellular biochemical response to the cryopreservation process, these media provide enhanced cell viability and functionality, while eliminating the need to include serum, proteins, or high levels of cytotoxic agents.

CryoStor CS2, CS5, and CS10 are a series of cell specific, optimized preservation media, uniquely formulated to address the molecular biological aspects of cells during the cryopreservation process; thereby, directly reducing the level of Cryopreservation-Induced Delayed-Onset Cell Death and improving post-thaw cell viability and function.

These media are recommended for the preservation of stem cells, hepatocytes, tissue samples, and other extremely sensitive cell types.


CryoStor® cell cryopreservation media has been used in:
  • the preservation of T cells
  • the preservation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)
  • hiPSC-derived cardiac progenitors (hiPSC-CPs)
  • as a component of cryoprotective solution composed of bovine albumin

CryoStor, a series of cell-specific, optimized preservation media, is uniquely formulated to address the molecularbiological aspects of cells during the cryopreservation process thereby directly reducing the level of Cryopreservation-Induced Delayed-Onset Cell Death and improving post-thaw cell viability and function.

Biochem/physiol Actions

CryoStor® CS10 is a uniquely formulated cryopreservation medium containing 10% dimethyl sulfoxide (DMSO). Recommended for the preservation of hepatocytes, tissue samples and other extremely sensitive cell types.

Cryostor® Cryopreservation Video Protocol

Other Notes

Formulation contains 10% DMSO.

Legal Information

CryoStor is a registered trademark of BioLife Solutions, Inc.



Signal Word


Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Physical events occurring during the cryopreservation of immortalized human T cells
Meneghel J, et al.
PLoS ONE, 14(5), e0217304-e0217304 (2019)
In vivo maturation of human induced pluripotent stem cell-derived cardiomyocytes in neonatal and adult rat hearts
Kadota S, et al.
Stem Cell Reports, 8(2), 278-289 (2017)
Huyen Thi Lam Nguyen et al.
STAR protocols, 1(2) (2020-10-13)
Tumor organoids are promising tools for cancer biology investigations and preclinical drug screenings because they are often representative of the histology and drug responses of patients. Here, we introduce a facile protocol to overcome technical limitations by generating patient-derived tumor...
The impact of varying cooling and thawing rates on the quality of cryopreserved human peripheral blood T cells
Baboo J, et al.
Scientific reports, 9(1), 3417-3417 (2019)
Dominic M Clarke et al.
Cytotherapy, 11(4), 472-479 (2009-06-06)
Peripheral blood stem cells (PBSC) have become the preferred stem cell source for autologous hematopoietic transplantation. A critical aspect of this treatment modality is cryopreservation of the stem cell products, which permits temporal separation of the PBSC mobilization/collection phase from...


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CryoStor® Cryopreservation Protocol

Cryopreservation efficacy which includes post-thaw recovery, viability, and functionality is of importance to both research and clinical applications. The cumulative stresses that result from the cryopreservation process and suboptimal freeze media result in cell death from necrosis and apoptosis.

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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