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C6164

Sigma-Aldrich

Cell Freezing Medium-DMSO 1×

sterile-filtered, suitable for cell culture

NACRES:
NA.75

sterility

sterile-filtered

Quality Level

form

liquid

application(s)

cell culture | mammalian: suitable
cryopreservation: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−20°C

General description

Cell freezing or cryopreservation of cell culture is a routine process. It is essential to maintain the cellular integrity during storage. Majorly used cryoprotectants include glycerol, ethylene glycol and dimethyl sulfoxide(DMSO).

Application

Cell Freezing Medium-DMSO 1× has been used in the cryopreservation of human embryonic kidney HEK293 CNR2 cells, blood mononuclear cells and prostate cancer cells.

Other Notes

Complete ready-to-use medium. Proprietary formulation with Minimum Essential Medium, dimethyl sulfoxide, calf serum and fetal bovine serum.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How much DMSO is present in Product C6164, Cell Freezing Medium-DMSO?

    The DMSO is at a 10% concentration.

  6. Does Product C6164, Cell Freezing Medium-DMSO, contain heat-inactivated serum? 

    No, the serum in this cell freezing media is not heat-inactivated.

  7. Is there insulin in Product C6164, Cell Freezing Medium-DMSO?

    No, there is no added insulin in this product.  There may be traces of insulin from the serum used in this product.

  8. Is it ok to sterile filter Product C6164, Cell Freezing Medium-DMSO?

    Product No. C6164 is sterile-filtered.  If the sterility of the product has been compromised, it can be re-filtered.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Antonia Patsialou et al.
Cancer research, 69(24), 9498-9506 (2009-11-26)
Colony-stimulating factor-1 (CSF-1) and its receptor (CSF-1R) have been implicated in the pathogenesis and progression of various types of cancer, including breast cancer. This is based on high levels of circulating CSF-1 in patient sera with aggressive disease and increased...
Safety and immunogenicity of oral microencapsulated enterotoxigenic Escherichia coli surface antigen 6 (CS6) with and without LTR192G: a randomized clinical trial
Lapa JA, et al.
Clinical and Vaccine Immunology : CVI (2008)
Variations of components of the plasminogen activation system with the cell cycle in benign prostate tissue and prostate cancer
Plas E, et al.
Cytometry, 46(3), 184-189 (2001)
Directional freezing for the cryopreservation of adherent mammalian cells on a substrate
Bahari L, et al.
Testing, 13(2), e0192265-e0192265 (2018)
Scott A Jackson et al.
BMC genomics, 12, 349-349 (2011-07-08)
The gene content of a diverse group of 183 unique Escherichia coli and Shigella isolates was determined using the Affymetrix GeneChip® E. coli Genome 2.0 Array, originally designed for transcriptome analysis, as a genotyping tool. The probe set design utilized...

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