All Photos(1)

C9249

Sigma-Aldrich

CryoSOfree DMSO-free Cryopreservation Medium

greener alternative
NACRES:
NA.75

form

liquid

Quality Level

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

application(s)

cell culture | mammalian: suitable
cryopreservation: suitable

greener alternative category

Aligned

shipped in

wet ice

storage temp.

2-8°C

General description

Cultured cells in a suspension can be cryopreserved with minimal damage from freezing and thawing steps. CryoSOfree DMSO-free cryopreservation medium preserves them with high viability and physiological activity. This medium is recommended for the preservation of stem cells, hepatocytes, tissue sample and a wide variety of cell types and established cell lines. CryoSOfree DMSO-free cryopreservation medium is a proprietary formulation, containing no DMSO nor animal proteins. It is ready-to-use and complete with no additives required.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of DMSO for cryopreservation of cell cultures. For more information see here.

Application

CryoSOfree is a chemically defined DMSO-free cryopreservation medium. It is animal component free and has been shown to preserve the potential of stem cell for differentiation.

Features and Benefits

  • Preserves pluripotency of stem cells
  • Low cytotoxicity
  • Chemically defined, serum free, protein free
  • Ready to use


The following cell lines have been recovered at ≥90% following cryopreservation with CryoSOfree:
L929, KB, MC3T3, B16F1, Colon26, CaCO2, HT1080, MG63

Preparation Note

1 ml of CyroSOfree is sufficient for 5x105- 5x106 cells.

Legal Information

CryoSOfree is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Kazuaki Matsumura et al.
Biomaterials, 30(27), 4842-4849 (2009-06-12)
Dimethyl sulfoxide (DMSO) has been used for several decades as the most efficient cryoprotective agent (CPA) for many types of cells and tissues in spite of its cytotoxicity and its effects on differentiation. Here we report that polyampholytes with an...
Kazuaki Matsumura et al.
Cell transplantation, 19(6), 691-699 (2010-06-08)
Cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO), glycerol, ethylene glycol, and propylene glycol have been used for the cryopreservation of cells and tissues. DMSO is the most effective CPA but shows high cytotoxicity and can effect differentiation. ɛ-poly-L-lysine (PLL)...

Related Content

Cell Therapy Manufacturing

Introducing a new cell therapy demands optimal performance in upstream and downstream processing, process development, testing, and manufacturing. World-class products and partners are critical to success, speeding advancement of therapeutics that are effective, safe, and affordable.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service