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D1408

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

10×, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
DPBS
MDL number:
eCl@ss:
32129211
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
glucose: no (Dextro)

shipped in

ambient

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General description

Dulbecco′s Phosphate Buffered Saline (DPBS) is a balanced salt solution.

Application

Dulbecco′s Phosphate Buffered Saline has been used:
  • in microfluidic cell culture of mouse embryonic stem cells (mESC)
  • to dissolve albumin for adsorption studies
  • in PC12 cell culture experiments

Dulbecco′s Phosphate Buffered Saline 10X is a stock solution used to prepare 1X D-PBS in cell culture grade water (W3500). D-PBS is used to wash cells during preparation and serial transfer.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Frank Klont et al.
Talanta, 182, 414-421 (2018-03-05)
The study of proteins is central to unraveling (patho)physiological processes and has contributed greatly to our understanding of biological systems. Corresponding studies often employ procedures to enrich proteins from their biological matrix using antibodies or other affinity binders coupled to...
Alexandre Super et al.
Biotechnology journal, 11(9), 1179-1189 (2016-05-24)
Oxygen plays a key role in stem cell biology as a signaling molecule and as an indicator of cell energy metabolism. Quantification of cellular oxygen kinetics, i.e. the determination of specific oxygen uptake rates (sOURs), is routinely used to understand...
Marjan Nabili et al.
Ultrasound in medicine & biology, 39(4), 638-646 (2013-02-19)
Delivery of sufficient amounts of therapeutic drugs into the eye is often a challenging task. In this study, ultrasound application (frequencies of 400 KHz to 1 MHz, intensities of 0.3-1.0 W/cm(2) and exposure duration of 5 min) was investigated to overcome...
A D Cabral et al.
The Analyst, 142(20), 3922-3933 (2017-09-21)
Chemosensors for the detection of phosphate-containing biological species are in high need. Detection of proximally phosphorylated sites of PP
Yuta Tanaka et al.
Journal of biomedical materials research. Part A, 92(1), 350-358 (2009-02-04)
Both terminals of a poly(ethylene glycol) (PEG) molecule were terminated with amines. The PEG was electrodeposited onto titanium (Ti) to give it the biofunctions such as blood compatibility. The effects of the amine of PEG terminals and the pH at...

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