Merck
All Photos(1)

D5030

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium

Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

All Photos(1)
Synonym(s):
DME, DMEM
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

Related Categories

Compare Similar Items

View Full Comparison

1 of 4

This Item
M3942BR10014BR10012
Sigma-Aldrich

Sigma-Aldrich

D5030

Dulbecco′s Modified Eagle′s Medium

SAFC

SAFC

M3942

MegaCell™ Dulbecco′s Modified Eagle′s Medium

BRAND® compact burette ′Economy′ SILBERBRAND capacity 50 mL, with PTFE stopcock and PP tip

BR10014

BRAND® compact burette ′Economy′ SILBERBRAND

BRAND® compact burette ′Economy′ SILBERBRAND capacity 25 mL, with PTFE stopcock and PP tip

BR10012

BRAND® compact burette ′Economy′ SILBERBRAND

form

powder

form

liquid

form

-

form

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

components

phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no

components

L-glutamine: no
glucose: 4500 mg/L (Dextro)
sodium pyruvate: yes

components

-

components

-

shipped in

ambient

shipped in

ambient

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

-

Quality Level

500

Quality Level

400

Quality Level

-

Quality Level

-

General description

Dulbecco′s Modified Eagle′s Medium (DME) is a modification of Basal Medium Eagle (BME) that has been formulated with a 4-fold higher concentration of amino acids and vitamins. It includes additional supplementary components. The DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. Modifying the medium with 4,500 mg/L glucose is optimal for culturing certain cell types.
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.

Application

Dulbecco′s Modified Eagle′s Medium has been used:
  • to culture the isolated flexor digitorum brevis (FDB) fibers to measure exogenous fatty acid (FA) utilization as part of oxygen consumption rate (OCR) measurements
  • to culture the isolated tibialis anterior (TA) muscle fibers for lactate measurements
  • to culture the human bone marrow mesenchymal stem cells for osteogenic differentiation to prepare a low-glucose medium to culture human hepatocellular carcinoma HepG2 cells

Quantity

Formulated to contain 8.3 grams of powder per liter of medium.

Reconstitution

Supplement with 1.0 g/L glucose, 0.584 g/L L-glutamine, 3.7 g/L sodium bicarbonate.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service