G0282

Granulocyte-Macrophage Colony-Stimulating Factor from mouse

GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): GM-CSF
• MDL number: MFCD00241918
• NACRES: NA.77

Properties

• Quality Level: 200
• biological source: mouse
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤0.2 ng/mL EC₅₀ (corresponds to ≥5 × 10⁶ units/mg)
• quality: endotoxin tested
• mol wt: ~15 kDa (125 amino acids including N-terminal methionine)
• packaging: pkg of 5 and 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/mg
• color: white
• solubility: water: soluble, clear, colorless
• UniProt accession no.: P01587
• storage temp.: −20°C
• Gene Information: mouse ... CSF2(12981) , Csf2(12981)

Description

Biochem/physiol Actions

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Physical form

Lyophilized from 10 mM acetic acid plus 250 μg BSA.

Analysis Note

The EC₅₀ activity of mouse GM-CSF is tested in culture using murine FDP-1 cells.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves