G9277

Guanidine thiocyanate

for molecular biology, ≥99%

• Synonym(s): Guanidinium rhodanide, Guanidinium thiocyanate
• Linear Formula: NH₂C(=NH)NH₂ · HSCN
• CAS Number: 593-84-0
• Molecular Weight: 118.16
• Beilstein: 3563461
• EC Number: 209-812-1
• MDL number: MFCD00013027
• PubChem Substance ID: 24895367
• NACRES: NA.31

Properties

• biological source: synthetic (organic)
• Quality Level: 200
• grade: for molecular biology
• assay: ≥99%
• form: powder or crystals
• technique(s): RNA extraction: suitable
• color: colorless to white,
• mp: 120-122 °C (lit.)
• solubility: water: 100 mg/mL, clear, colorless
• cation traces: Ba: ≤5 ppm; Fe: ≤5 ppm; Na: ≤0.5%; heavy metals (as Pb): ≤5 ppm
• application(s): agriculture
• foreign activity: DNase, RNase, none detected
• SMILES string: SC#N.NC(N)=N
• InChI: 1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
• InChI key: ZJYYHGLJYGJLLN-UHFFFAOYSA-N

Description

Application

Guanidine thiocyanate has been used:

• for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation
• as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay
• for DNA extraction from cerebrospinal fluid (CSF)

Chaotropic agent and strong denaturant; solubilizes cells.

Packaging

100, 250, 500, 500 g

1 kg

Biochem/physiol Actions

Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction.

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H302 + H312 + H332 - H314 - H412
• Precautionary Statements: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
• Supplementary Hazards: EUH032
• Storage Class Code: 8B - Non-combustible, corrosive hazardous materials
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves