H1136

Hyaluronidase from Streptomyces hyalurolyticus

• Synonym(s): Hyaluronate Lyase from Streptomyces hyalurolyticus
• CAS Number: 37259-53-3
• Enzyme Commission number: 4.2.2.1 (BRENDA, IUBMB)
• EC Number: 253-430-8
• MDL number: MFCD00131347
• NACRES: NA.54

Properties

• biological source: Streptomyces sp. (S. hyalurolyticus)
• Quality Level: 200
• form: film (Clear)
• packaging: ampule of 300 × units
• storage temp.: −20°C

Description

General description

Hyaluronidase is widely present in nature. In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards.

Application

Hyaluronidase from Streptomyces hyalurolyticus has been used:

• in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue
• as a component of digestion solution for the derivation of germline stem cells from testicular tissue

Packaging

1 amp

1 ampoules

Biochem/physiol Actions

Hyaluronate lyase cleaves hyaluronic acid at the β-D-GlcNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is inactive with chondroitin and chondroitin sulfate.

Hyaluronidase is an endoglycosidase and performs its function along with exoglycosidases, like β-glucuronidase and β-N-acetyl glucosaminidase. Hyaluronidase regulates the hyaluronic acid turnover.

Unit Definition

One unit will cause a change in % transmittance at 600 nm of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 mL reaction mixture (45 minute assay).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves