H3274
Hygromycin B from Streptomyces hygroscopicus
powder, non-animal origin, suitable for cell culture and insect cell culture, BioReagent
Recommended Products
biological source
Streptomyces hygroscopicus
Quality Level
product line
BioReagent
form
powder
concentration
≥60% (HPLC)
technique(s)
cell culture | insect: suitable
cell culture | mammalian: suitable
color
faintly brown to brown
white to beige
(1) 7.1, (2) 8.8
mp
160-180 °C
solubility
H2O: 50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 30 days.)
antibiotic activity spectrum
fungi
Mode of action
protein synthesis | interferes
storage temp.
2-8°C
SMILES string
CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
InChI
1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
InChI key
GRRNUXAQVGOGFE-XKIAHZFYSA-N
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General description
Application
- transfected 293T cells with mixture of plasmids at 160 μg/ml.
- MCF-7ALOX12 cells at 150 μg/ml.
- MSCV Luciferase PGK-hygro plasmid.
- STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS.
Biochem/physiol Actions
Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.
Caution
Preparation Note
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Articles
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Protocols
Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.
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