H3631

Hyaluronidase from bovine testes

Type VI-S, lyophilized powder, 3,000-15,000 units/mg solid

• Synonym(s): Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
• CAS Number: 37326-33-3
• Enzyme Commission number: 3.2.1.35 (BRENDA, IUBMB)
• EC Number: 253-464-3
• MDL number: MFCD00131351
• NACRES: NA.54

Properties

• biological source: bovine testis
• Quality Level: 200
• type: Type VI-S
• form: lyophilized powder
• specific activity: 3,000-15,000 units/mg solid
• mol wt: ~55 kDa (four subunits of 14 kDa each)
• UniProt accession no.: Q5E985; Q8SQG8
• relevant disease(s): cancer
• storage temp.: −20°C
• Gene Information: cow ... HYAL1(515397) , HYAL2(281838)

Description

Packaging

3000, 15000, 30000 units in glass bottle

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will cause a change in % transmittance at 600 nm of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 mL reaction mixture (45 minute assay).

Preparation Note

Chromatographically purified

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves