H9250

Histone from calf thymus

Type II-A, lyophilized powder

• Synonym(s): histone type VIII-S
• CAS Number: 9064-47-5
• Beilstein: 1895465
• MDL number: MFCD00131329
• NACRES: NA.51

Properties

• biological source: bovine (calf) thymus
• type: Type II-A
• form: lyophilized powder
• application(s): cell analysis
• storage temp.: 2-8°C

Description

General description

Histones are octameric proteins containing H3, H4, H2A and H2B. It is the core protein around which DNA is wound forming the nucleosome structure. The core histone proteins contain globular domains. Each protein is composed of basic amino acids at its N-terminal. The H2A has an extra amino acid at its C-terminal end forming a histone tail. The nucleosome structure is stabilized by the linker histones during chromatin condensation.

Application

Histone from calf thymus has been used:

• in methyltransferase reaction mixture for methyltransferase assay
• in in vitro binding assay, to explore the potential binding of the MORF4 (mortality factor on chromosome 4) - related gene on chromosome 15 (MRG15) chromo domain with histone
• in in vitro coculture assays, to stimulate liver resident Kupffer cells

Biochem/physiol Actions

The core histones are involved in histone-histone interactions and maintains the organization of the nucleosomal DNA. The histone tail helps in forming higher-order nucleosomal structures. The overall structure and action of chromatin fiber is regulated by histone modifications.

Features and Benefits

Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.

Preparation Note

Unfractionated whole histone

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable