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I1146

Sigma-Aldrich

R3 IGF-1 human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

≥85% (HPLC)

form

lyophilized powder

potency

≤10 ng/mL ED50

quality

endotoxin tested

mol wt

7,676 Da

packaging

pkg of 1 mg

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

human ... IGF1(3479)

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This Item
SRP3069I3769F5542
Sigma-Aldrich

Sigma-Aldrich

I1146

R3 IGF-1 human

Sigma-Aldrich

Sigma-Aldrich

SRP3069

IGF-I human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥85% (HPLC)

assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥97% (SDS-PAGE)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

potency

≤10 ng/mL ED50

potency

0.1-10 ng/mL ED50/EC50

potency

0.1-10 ng/mL ED50/EC50

potency

0.287 ng/mL

mol wt

7,676 Da

mol wt

~7.6 kDa

mol wt

~7.6 kDa

mol wt

15.5 kDa

Application

R3 IGF-I is a recombinant analog of insulin-like growth factor containing the complete human IGF-I amino acid sequence with substitution of Arg for Glu3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture.

Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.

Biochem/physiol Actions

Product stimulates protein synthesis in L6 myoblasts.

Other Notes

Recombinant analog of insulin-like growth factor-I with the substitution of Arg for Glu at position 3.

Preparation Note

Prepared from 0.1 M acetic acid

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Insulin-like Growth Factors (IGF)

Insulin-like Growth Factors (IGF)

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