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L5158

Sigma-Aldrich

Leukemia Inhibitory Factor from mouse

LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution, suitable for cell culture

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Synonym(s):
Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, mLIF, LIF
MDL number:
MFCD00283681
NACRES:
NA.77

recombinant

expressed in E. coli

Quality Level

200

Assay

≥95% (SDS-PAGE)

form

buffered aqueous solution

quality

endotoxin tested

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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This Item
LIF2010LIF2005L5283
Leukemia Inhibitory Factor from mouse LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution, suitable for cell culture

Sigma-Aldrich

L5158

Leukemia Inhibitory Factor from mouse

Sigma-Aldrich

Sigma-Aldrich

LIF2010

Leukemia Inhibitory Factor from mouse

Sigma-Aldrich

Sigma-Aldrich

LIF2005

Leukemia Inhibitory Factor from mouse

Leukemia Inhibitory Factor human LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution (pH 7.4), suitable for cell culture

Sigma-Aldrich

L5283

Leukemia Inhibitory Factor human

assay

≥95% (SDS-PAGE)

assay

>95% (HPLC and SDS-PAGE)

assay

>95% (HPLC and SDS-PAGE)

assay

≥95% (SDS-PAGE)

form

buffered aqueous solution

form

liquid

form

liquid

form

buffered aqueous solution (pH 7.4)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

2-8°C

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

200

Application

Leukemia Inhibitory Factor from mouse has been used:
  • as a component of enriched co-culture media
  • as a supplement in Dulbecco′s modified eagle′s medium (DMEM)/F12 medium for culturing heart cells
  • as a component in maintenance medium for maintaining embryonic stem cells
  • to treat ovaries in ovarian culture

Biochem/physiol Actions

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

Physical form

Solution in phosphate buffered saline containing 0.02% TWEEN® 20

Analysis Note

The proliferative activity of mouse LIF is measured in culture using the M1 murine cell line.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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C-Kit Positive Cardiac Stem Cells and Bone Marrow-Derived Mesenchymal Stem Cells Synergistically Enhance Angiogenesis and Improve Cardiac Function After Myocardial Infarction in a Paracrine Manner
Bao L, et al.
Journal of Cardiac Failure, 23(5), 403-415 (2017)
Suppressor of cytokine signaling 4 (SOCS4): moderator of ovarian primordial follicle activation
Sutherland JM, et al.
Journal of Cellular Physiology, 227(3), 1188-1198 (2012)
LIF-immobilized nonwoven polyester fabrics for cultivation of murine embryonic stem cells
Cetinkaya G, et al.
Journal of Biomedical Materials Research Part A, 81(4), 911-919 (2007)
Renée F de Pooter et al.
Methods in molecular biology (Clifton, N.J.), 330, 113-121 (2006-07-19)
Mature hematopoietic cells, like all other terminally differentiated lineages, arise during ontogeny via a series of increasingly restricted intermediates. Hematopoietic progenitors have their origin in the mesoderm, which gives rise to hemangioblasts that can differentiate into endothelial or endocardial precursors
Cristian Soitu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(23), 2001854-2001854 (2020-12-12)
There is an unmet demand for microfluidics in biomedicine. This paper describes contactless fabrication of microfluidic circuits on standard Petri dishes using just a dispensing needle, syringe pump, three-way traverse, cell-culture media, and an immiscible fluorocarbon (FC40). A submerged microjet
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Articles

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

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