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L9545

Sigma-Aldrich

Leukemia Inhibitory Factor human, animal component free

recombinant, expressed in rice, 500 μg/mL

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Synonym(s):
Leukemia Inhibitory Factor human, LIF, rLIF
MDL number:
NACRES:
NA.32

recombinant

expressed in rice

Quality Level

Assay

>95% (SDS-PAGE)

potency

>1 x 108 units/mg ED<sub>50</sub>

concentration

500 μg/mL

technique(s)

cell culture | mammalian: suitable

impurities

<0.05 EU/μg endotoxin

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... LIF(3976)

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This Item
LIF1050LIF1010LIF2010
assay

>95% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

>95% (HPLC and SDS-PAGE)

assay

>95% (HPLC and SDS-PAGE)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

impurities

<0.05 EU/μg endotoxin

impurities

<0.1 ng/mg Endotoxin (of LIF)

impurities

<0.1 ng/mg Endotoxin (of LIF)

impurities

<0.1 ng/mg Endotoxin (of LIF)

UniProt accession no.

P15018

UniProt accession no.

P15018

UniProt accession no.

P15018

UniProt accession no.

P09056

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

Biochem/physiol Actions

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

Physical form

0.2 μm filtered solution at 500 μg/mL in buffered saline with 0.02% TWEEN®20

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

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