M2909

Murashige and Skoog Modified Basal Salt Mixture

powder, suitable for plant cell culture

• Synonym(s): Murashige and Skoog Modified Basal Salt Mixture, w/o Ammonium Nitrate
• NACRES: NA.72

Properties

• form: powder
• Quality Level: 200
• technique(s): cell culture | plant: suitable
• application(s): agriculture
• storage temp.: 2-8°C

Description

Application

Murashige and Skoog Modified Basal Salt Mixture has been used:

• to promote root development for internode stem suckers
• to promote shoot growth for the calluses
• for low nitrogen (LN) treatment, for the growth of Arabidopsis thaliana

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Formula variant

Without NH₄NO₃
With the macro- and micronutrients described by Murashige and Skoog (1962).

Media Formulation

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M2909) contain the macronutrients and micronutrients of the original classic formulation without ammonium nitrate. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Safety Information

• Pictograms: GHS03,GHS07
• Signal Word: Warning
• Hazard Statements: H272 - H319
• Precautionary Statements: P210 - P220 - P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Ox. Sol. 3
• Storage Class Code: 5.1B - Oxidizing hazardous materials
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable