Merck
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M5017

Sigma-Aldrich

Medium 199

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
L-glutamine: yes
Earle’s salts (5% CO2): yes
phenol red: yes
HEPES: no
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

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General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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