This surfactant is soluble in water at >10% at room temperature. The product solubility is tested at 50 mg/mL in water. Similar rates are expected in PBS. The solubility in DMSO has not been investigated.
P2443
Pluronic® F-127
powder, BioReagent, suitable for cell culture
Synonym(s):
Non-ionic copolymer surfactant
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description
Non-ionic
contains 100ppm BHT
Quality Level
product line
BioReagent
form
powder
mol wt
~12600 g/mol
technique(s)
cell culture | mammalian: suitable
CMC
950-1000 ppm (~25°C)
transition temp
cloud point >100 °C
HLB
18 - 23
SMILES string
O2C(C2)C.O1CC1
InChI
1S/C3H6O.C2H4O/c1-3-2-4-3;1-2-3-1/h3H,2H2,1H3;1-2H2
InChI key
RVGRUAULSDPKGF-UHFFFAOYSA-N
Related Categories
1 of 4
This Item | 07579 | 86216 | P4894 |
---|---|---|---|
technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) cell culture | mammalian: suitable |
form powder | form - | form solid | form solid, waxy solid |
CMC 950-1000 ppm (~25°C) | CMC - | CMC - | CMC - |
product line BioReagent | product line - | product line - | product line - |
HLB 18 - 23 | HLB - | HLB - | HLB - |
mol wt ~12600 g/mol | mol wt - | mol wt - | mol wt - |
General description
Application
- as a delivery system for a peptide to the wound site to study the effect of the peptide on wound healing in mice.[4]
- as a surface coating for PDMS (polydimethylsiloxane) stamps to make them hydrogel repellent.[5]
- in the preparation of calcium indicator stock solution for measuring neural activities.[6]
- as a component of phosphate-buffered saline (PBS), to lower unspecific cell and protein adhesion to a PDMS-based microfluidic device.[7]
- This non-ionic copolymer surfactant is suitable for use in insect cell culture applications as an antifoaming agent.
Biochem/physiol Actions
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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Pluroni F-127 어떤 solution에 녹여서 stock 만들어야 되나요? D.W 인가요? DMSO 인가요? PBS 인가요?
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Is Pluronic® F-127 soluble in water and/or KOH solutions (up to 10M)?
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This product is tested for solubility at 50 mg/mL in water at temperatures less than 10°C. Maximum solubility has not been tested.
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What is the hydroxyl number of F127?
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Based on an approximate molecular weight of 12,600, this block co-polymer with two hydroxyl groups has a theoretical hydroxyl value of 8.9 mg potassium hydroxide (KOH) per gram of polymer. Higher experimental results for the hydroxyl value would indicate some branching.
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Is there a reference for using Pluronic F-127 to reduce cell adhesion in a microfluidic PDMS-based device? Also, does it dissolve in cell culture media in a PDMS-based device, and if it does, what modifications are recommended to maintain its ability to prevent cell adhesion?
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There are several representative publications that report the use of Pluronic F-127 to lower cell adhesion on microfluidic PDMS-based devices. These publications may be of interest as a reference:
Curr Protoc Cell Biol. 2017 Jun 19;75:10.21.1-10.21.8. Microcontact Peeling: A Cell Micropatterning Technique for Circumventing Direct Adsorption of Proteins to Hydrophobic PDMS. PMID: 28627756
Sci Rep. 2019 May 14;9(1):7377. Simple Surface Modification of Poly(dimethylsiloxane) via Surface Segregating Smart Polymers for Biomicrofluidics. PMID: 31089162
Lab Chip. 2009 Jun 7;9(11):1500-3. Surface modification of PDMS by gradient-induced migration of embedded Pluronic. PMID: 19458853
These publications provide information on using Pluronic F-127 to lower cell adhesion on PDMS-based devices. As for its solubility in cell culture media within a PDMS-based device and the recommended modifications to maintain its ability to prevent cell adhesion, it is advised to refer to these publications for detailed insights.
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I was asked about the polydispersity of Pluronic F-127, Product P2443. Could you please inform me?
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The polydispersity is not a test specification for this product. The product is an ABA-type copolymer of poly (ethylene oxide) (PEO=A) and poly (propylene oxide) (PPO=B), where the A and B blocks have the following values: A = 101, B = 56. The approximate relative amount of PEO in the poloxamer is ca. 70% and the average molecular weight of the PPO is 4000. The molecular structure is shown below:
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can I know what is the chain length of each PEO and PPO of this F-127?
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Pluronic F 127 corresponds to the structure of Kolliphor P 407.
This product is an ABA-type copolymer of polyethylene oxide (PEO=A) and polypropylene oxide (PPO=B), where the A and B blocks have the following values:
A = 101
B = 56Helpful?
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What is the VOC content of this product?
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Volatile Organic Compound (VOC) content is not measured for this product.
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Une fois le Pluronic® F-127 mis en solution (à 10% dans de l'eau par exemple), combien de temps et à quelle température se conserve-t-il ?
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The solubility of the product is 50 mg/ml water, at less than 10°C. The stability of the product once diluted has not been tested, in which case it is suggested to prepare the necessary quantity before use.
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What is the best way to sterilise Pluronic F127 dissolved in water so that it is suitable for cell culture?
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The solution can be sterilized with a 0.22 uM sterile filter.
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Is corn oil soluble in Pluronic PBS gel?
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In general, Pluronic acid products are poor solubilizers of oils. However, they may make a good dispersant or emulsifier for corn oil.
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