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Merck

P2443

Sigma-Aldrich

Pluronic® F-127

powder, BioReagent, suitable for cell culture

Synonym(s):

Non-ionic copolymer surfactant

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250 G
£77.90
1 KG
£249.00

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250 G
£77.90
1 KG
£249.00

About This Item

Linear Formula:
(C3H6O·C2H4O)x
CAS Number:
MDL number:
UNSPSC Code:
12161902
PubChem Substance ID:
NACRES:
NA.75

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£77.90


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description

Non-ionic
contains 100ppm BHT

Quality Level

product line

BioReagent

form

powder

mol wt

~12600 g/mol

technique(s)

cell culture | mammalian: suitable

CMC

950-1000 ppm (~25°C)

transition temp

cloud point >100 °C

HLB

18 - 23

SMILES string

O2C(C2)C.O1CC1

InChI

1S/C3H6O.C2H4O/c1-3-2-4-3;1-2-3-1/h3H,2H2,1H3;1-2H2

InChI key

RVGRUAULSDPKGF-UHFFFAOYSA-N

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This Item
0757986216P4894
technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

cell culture | mammalian: suitable

form

powder

form

-

form

solid

form

solid, waxy solid

CMC

950-1000 ppm (~25°C)

CMC

-

CMC

-

CMC

-

product line

BioReagent

product line

-

product line

-

product line

-

HLB

18 - 23

HLB

-

HLB

-

HLB

-

mol wt

~12600 g/mol

mol wt

-

mol wt

-

mol wt

-

General description

Pluronicpluronic® F-127, also known as poloxamer 407, is a non-toxic[1] and nonionic copolymer. It contains polyethylene oxide-polypropylene oxide arranged in a triblock structure to form PEO-PPO-PEO symmetric.[2] Surfactant properties of this polymer derived from its amphiphilic nature, allow them to interact with hydrophobic surfaces and biological membranes. [3]

Application

Pluronic® F-127 has been used:

  • as a delivery system for a peptide to the wound site to study the effect of the peptide on wound healing in mice.[4]
  • as a surface coating for PDMS (polydimethylsiloxane) stamps to make them hydrogel repellent.[5]
  • in the preparation of calcium indicator stock solution for measuring neural activities.[6]
  • as a component of phosphate-buffered saline (PBS), to lower unspecific cell and protein adhesion to a PDMS-based microfluidic device.[7]
  • This non-ionic copolymer surfactant is suitable for use in insect cell culture applications as an antifoaming agent.

Biochem/physiol Actions

Pluronic® F-127 is a thermo-reversible gel that finds its application in different industries due to its high thermosensitivity, biodegradability, and biocompatibility properties.[1] These properties make this polymer suitable for use as a versatile drug carrier.Pluronic® F-127 improves the therapeutic efficiency of drugs by increasing their bioavailability and thermostability.[8] Additionally, it can promote cellular attachment and collagen formation, which facilitates angiogenesis and tissue regeneration. Due to its efficacy, Pluronic F-127 has been utilized in the encapsulation of mesenchymal stem cells (MSCs) to aid in the regeneration of tissues with low levels of vascularity, such as epithelial tissues, adipose tissues, cartilage, tendons, and bone tissues.[1]

Legal Information

Pluronic is a registered trademark of BASF

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Questions

1–10 of 16 Questions  
  1. Pluroni F-127 어떤 solution에 녹여서 stock 만들어야 되나요? D.W 인가요? DMSO 인가요? PBS 인가요?

    1 answer
    1. This surfactant is soluble in water at >10% at room temperature. The product solubility is tested at 50 mg/mL in water. Similar rates are expected in PBS. The solubility in DMSO has not been investigated.

      Helpful?

  2. Is Pluronic® F-127 soluble in water and/or KOH solutions (up to 10M)?

    1 answer
    1. This product is tested for solubility at 50 mg/mL in water at temperatures less than 10°C. Maximum solubility has not been tested.

      Helpful?

  3. What is the hydroxyl number of F127?

    1 answer
    1. Based on an approximate molecular weight of 12,600, this block co-polymer with two hydroxyl groups has a theoretical hydroxyl value of 8.9 mg potassium hydroxide (KOH) per gram of polymer. Higher experimental results for the hydroxyl value would indicate some branching.

      Helpful?

  4. Is there a reference for using Pluronic F-127 to reduce cell adhesion in a microfluidic PDMS-based device? Also, does it dissolve in cell culture media in a PDMS-based device, and if it does, what modifications are recommended to maintain its ability to prevent cell adhesion?

    1 answer
    1. There are several representative publications that report the use of Pluronic F-127 to lower cell adhesion on microfluidic PDMS-based devices. These publications may be of interest as a reference:

      Curr Protoc Cell Biol. 2017 Jun 19;75:10.21.1-10.21.8. Microcontact Peeling: A Cell Micropatterning Technique for Circumventing Direct Adsorption of Proteins to Hydrophobic PDMS. PMID: 28627756

      Sci Rep. 2019 May 14;9(1):7377. Simple Surface Modification of Poly(dimethylsiloxane) via Surface Segregating Smart Polymers for Biomicrofluidics. PMID: 31089162

      Lab Chip. 2009 Jun 7;9(11):1500-3. Surface modification of PDMS by gradient-induced migration of embedded Pluronic. PMID: 19458853

      These publications provide information on using Pluronic F-127 to lower cell adhesion on PDMS-based devices. As for its solubility in cell culture media within a PDMS-based device and the recommended modifications to maintain its ability to prevent cell adhesion, it is advised to refer to these publications for detailed insights.

      Helpful?

  5. I was asked about the polydispersity of Pluronic F-127, Product P2443. Could you please inform me?

    1 answer
    1. The polydispersity is not a test specification for this product. The product is an ABA-type copolymer of poly (ethylene oxide) (PEO=A) and poly (propylene oxide) (PPO=B), where the A and B blocks have the following values: A = 101, B = 56. The approximate relative amount of PEO in the poloxamer is ca. 70% and the average molecular weight of the PPO is 4000. The molecular structure is shown below:

      Helpful?

  6. can I know what is the chain length of each PEO and PPO of this F-127?

    1 answer
    1. Pluronic F 127 corresponds to the structure of Kolliphor P 407.
      This product is an ABA-type copolymer of polyethylene oxide (PEO=A) and polypropylene oxide (PPO=B), where the A and B blocks have the following values:
      A = 101
      B = 56

      Helpful?

  7. What is the VOC content of this product?

    1 answer
    1. Volatile Organic Compound (VOC) content is not measured for this product.

      Helpful?

  8. Une fois le Pluronic® F-127 mis en solution (à 10% dans de l'eau par exemple), combien de temps et à quelle température se conserve-t-il ?

    1 answer
    1. The solubility of the product is 50 mg/ml water, at less than 10°C. The stability of the product once diluted has not been tested, in which case it is suggested to prepare the necessary quantity before use.

      Helpful?

  9. What is the best way to sterilise Pluronic F127 dissolved in water so that it is suitable for cell culture?

    1 answer
    1. The solution can be sterilized with a 0.22 uM sterile filter.

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  10. Is corn oil soluble in Pluronic PBS gel?

    1 answer
    1. In general, Pluronic acid products are poor solubilizers of oils. However, they may make a good dispersant or emulsifier for corn oil.

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1–10 of 16 Questions  

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