All Photos(3)

P2443

Sigma-Aldrich

Pluronic® F-127

powder, BioReagent, suitable for cell culture

Linear Formula:
(C3H6O·C2H4O)x
CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

Quality Level

description

Non-ionic
contains 100ppm BHT

product line

BioReagent

form

powder

mol wt

~12600 g/mol

application(s)

cell culture | mammalian: suitable

CMC

950-1000 ppm (~25°C)

transition temp

cloud point >100  °C

HLB

18 - 23

InChI

1S/C3H6O.C2H4O/c1-3-2-4-3;1-2-3-1/h3H,2H2,1H3;1-2H2

InChI key

RVGRUAULSDPKGF-UHFFFAOYSA-N

Related Categories

Application

A non-ionic copolymer surfactant qualified for use in insect cell culture applications as an antifoaming agent.
Pluronic® F-127 was used to coat a siliconized coverslip to hold an egg extract in a study. Pluronic F-127 was added to phosphate buffered saline (PBS), to lower unspecific cell and protein adhesion to a PDMS-based microfluidic device. A study reports its use as a release vehicle to transport low-dose perivascular lipopolysaccharide (LPS) on mouse vein grafts. PLGA/pluronic F127 may be used to fabricate nerve guidance conduits (NGCs) for regeneration of peripheral nerve. Fabrication of poly (lactide-co-glycolide) (PLGA) — Pluronic F127 glass composites was reported. Pluronic F-127 was used for fluorescent labeling of blood vessels, astrocytes, and neurons.

Packaging

250 g in poly bottle
1 kg in poly bottle

Legal Information

Pluronic is a registered trademark of BASF

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the molecular weight of Pluronic F-127, Product P2443?

    The average molecular weight is 12.6 kDa.

  4. Why has my solution of Pluronic F-127, Product P2443, gelled?

    Pluronic F-127 will form gels above 10 °C when used at concentrations of 18-50%. It will re-liquefy when cooled to below 10 °C. The gel should remain stable during autoclaving.

  5. What concentration of Pluronic F-127, Product P2443, should be used in cell culture?

    We test Pluronic F-127 in cell culture at 0.1 g/100 ml media (0.1%).  The concentration necessary for your cell application may vary.

  6. What can I use to dilute Pluronic F-127, Product P2443?

    The Pluronic F-127 is water soluble as stated on the Specification Sheet.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Federico Ceriani et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(46), E7194-E7201 (2016-11-04)
Spatially and temporally coordinated variations of the cytosolic free calcium concentration ([Ca2+]c) play a crucial role in a variety of tissues. In the developing sensory epithelium of the mammalian cochlea, elevation of extracellular adenosine trisphosphate concentration ([ATP]e) triggers [Ca2+]c oscillations...
Philippe Marmottant et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(41), 17271-17275 (2009-10-07)
Cell aggregates are a tool for in vitro studies of morphogenesis, cancer invasion, and tissue engineering. They respond to mechanical forces as a complex rather than simple liquid. To change an aggregate's shape, cells have to overcome energy barriers. If...
P Guillamat et al.
Nature communications, 8(1), 564-564 (2017-09-17)
Active matter embraces systems that self-organize at different length and time scales, often exhibiting turbulent flows apparently deprived of spatiotemporal coherence. Here, we use a layer of a tubulin-based active gel to demonstrate that the geometry of active flows is...
Bana Shriky et al.
Journal of colloid and interface science, 565, 119-130 (2020-01-17)
Understanding structure-property relationships is critical for the development of new drug delivery systems. This study investigates the properties of Pluronic smart hydrogel formulations for future use as injectable controlled drug carriers. The smart hydrogels promise to enhance patient compliance, decrease...
Veronica Zorzi et al.
Frontiers in molecular neuroscience, 10, 379-379 (2017-12-14)
Panx1 forms plasma membrane channels in brain and several other organs, including the inner ear. Biophysical properties, activation mechanisms and modulators of Panx1 channels have been characterized in detail, however the impact of

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