Merck
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P2714

Sigma-Aldrich

Protease Inhibitor Cocktail powder

for general use, lyophilized powder

Synonym(s):
Protease inhibitor
NACRES:
NA.77

biological source

bovine lung

Quality Level

form

lyophilized powder

storage temp.

−20°C

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Specificity

Inhibits serine, cysteine, and metallo-proteases.

Packaging

1 bottle in serum bottle

Application

Optimized and tested for general use.

Biochem/physiol Actions

This mixture contains individual components, including AEBSF at 2 mM, Aprotinin at 0.3 μM, Bestatin at 116 μM, E-64 at 14 μM, Leupeptin at 1 μM and EDTA at 1 mM. Each component has specific inhibitory properties.

AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.

Components

AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin

Caution

The lyophilized powder is stable for 4 years when stored at -20°C.

Quantity

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Preparation Note

This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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