P8169

Phytagel^™

BioReagent, suitable for plant cell culture, powder

• Synonym(s): Gellan Gum, Agar substitute gelling agent
• CAS Number: 71010-52-1
• EC Number: 275-117-5
• UNSPSC Code: 10171502
• NACRES: NA.72

Properties

• product line: BioReagent
• Quality Level: 200
• form: powder
• technique(s): cell culture | plant: suitable
• application(s): agriculture

Description

General description

Typical working concentration: 1.5-2.5 g/L in plant tissue culture media; up to 10 g/L in microbiological media. Phytagel^™ requires the presence of cations (especially divalent) for gelling to occur. Concentrations of calcium and magnesium contained in most plant tissue culture media are typically sufficient for gelation. Low-salt media formulations, especially those used in microbiological applications, may require supplementation with additional calcium or magnesium salts (e.g., CaCl₂ or MgSO₄) or higher concentrations of Phytagel.

Application

Phytagel^™ has been used:

• in the solidification of culture medium for root assays in Arabidopsis thaliana[1]
• as a component of Solid Paul′s medium for Agrobacterium culture[2]
• as a component of root induction medium in Ilex dumosa tissue culture[3]

Biochem/physiol Actions

Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.

Other Notes

An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.

Reconstitution

To prevent clumping, add slowly to rapidly stirring medium prior to heating.

Legal Information

Phytagel is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves