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R4775

Sigma-Aldrich

REDExtract-N-Amp PCR ReadyMix

Ready-to-use 2X PCR Master Mix with Loading Dye

NACRES:
NA.55

Quality Level

usage

sufficient for 100 reactions
1.2 mL sufficient for 100 amplifications
sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications
sufficient for 10000 reactions
125 mL sufficient for 10000 amplifications

feature

dNTPs included
hotstart

color

red

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

General description

The REDExtract-N-Amp PCR reaction mix is a PCR Master mix containing buffer, salts, dNTPs, and REDTaq DNA polymerase.
This PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits are sufficient for one PCR amplification per extraction. However, where additional PCR reactions are required, this supplemental PCR ReadyMix may be needed. The REDExtract-N-Amp PCR ReadyMix contains an inert tracking dye that allows for convenient direct loading of PCR products onto agarose gels for analysis.

Application

REDExtract-N-Amp PCR Master Mix has been used for the genotyping of fixed zebrafish embryo samples. It has been used in PCR amplification of the DNA isolated from lichen family using the REDExtract-N-Amp Plant PCR Kit. It has also been used in the amplification of DNA isolated from single yeast colonies and fungal spores using REDExtract-N-Amp seed PCR kit.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Parallel evolution and phenotypic divergence in lichenized fungi: a case study in the lichen-forming fungal family Graphidaceae (Ascomycota: Lecanoromycetes: Ostropales).
Rivas Plata E
Molecular Phylogenetics and Evolution, 61(1), 45-63 (2011)
Rasmus H Jensen et al.
Journal of clinical microbiology, 49(2), 549-556 (2010-12-15)
Candida palmioleophila has previously been misidentified as C. famata or C. guilliermondii. We have investigated traditional and modern identification methods for the identification of this and related species. Forty-one clinical isolates previously identified as C. famata or C. guilliermondii and
Darinka Gjorgieva et al.
BioMed research international, 2013, 276417-276417 (2013-07-19)
Heavy metals have the potential to interact and induce several stress responses in the plants; thus, effects of heavy metal stress on DNA damages and total antioxidants level in Urtica dioica leaves and stems were investigated. The samples are sampled
Targeted mutagenesis in the progeny of maize transgenic plants.
Yang M
Plant Molecular Biology, 70(6), 669-679 (2009)
Ventral tegmental area dopamine cell activation during male rat sexual behavior regulates neuroplasticity and d-amphetamine cross-sensitization following sex abstinence
Beloate LN, et al.
The Journal of Neuroscience, 36, 9949-9961 (2016)

Articles

Extract-N-Amp™ Tissue Feature Article

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit

Extract-N-Amp™ Tissue Feature Article

Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.

Protocols

REDExtract-N-Amp™ Plant PCR Kit Protocol

The REDExtract-N-Amp Plant PCR kit includes an inert loading dye in the ReadyMix for easy gel loading after amplification. This protocol provides a rapid protocol to extract plant genomic DNA for amplification using the optimized ReadyMix.

REDExtract-N-Amp™ Tissue PCR Kit Protocol

The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.

Related Content

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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