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RAB0406

Sigma-Aldrich

Human Trappin-2 / Elafin  ELISA Kit

for serum, plasma, cell culture supernatant and urine

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NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 6.86-5000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PI3(5266)

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RAB0457RAB0472RAB0027
packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

input

sample type urine
sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type urine
sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type cell culture supernatant(s)
sample type serum
sample type urine
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 6.86-5000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20 pg/mL
standard curve range: 20.58-15000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 1.6-1200 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 ng/mL
standard curve range: 1.02-250 ng/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The Human Trappin-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Trappin-2 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human TRAPPIN2

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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25G
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Paula Tejera et al.
American journal of respiratory cell and molecular biology, 51(2), 262-272 (2014-03-13)
Elafin (peptidase inhibitor 3 [PI3]) and its biologically active precursor, pre-elafin, are neutrophil serine proteinase inhibitors with an important role in preventing excessive tissue injury during inflammatory events. Recently, we reported an association between single-nucleotide polymorphism (SNP) rs2664581 in the
Heather J Galipeau et al.
The American journal of gastroenterology, 109(5), 748-756 (2014-04-09)
Elafin, an endogenous serine protease inhibitor, modulates colonic inflammation. We investigated the role of elafin in celiac disease (CD) using human small intestinal tissues and in vitro assays of gliadin deamidation. We also investigated the potential beneficial effects of elafin
Sangeeta Hingorani et al.
Clinical journal of the American Society of Nephrology : CJASN, 10(1), 12-20 (2014-11-13)
Graft-versus-host disease (GVHD) is associated with kidney injury after hematopoietic cell transplantation (HCT). Because plasma elafin levels correlate with skin GVHD, this study examined urinary elafin as a potential marker of renal inflammation and injury. Urine was collected prospectively on
S I Labidi-Galy et al.
Oncogene, 34(3), 373-383 (2014-01-29)
High-grade serous ovarian carcinoma (HGSOC) and basal-like breast cancer (BLBC) share many features including TP53 mutations, genomic instability and poor prognosis. We recently reported that Elafin is overexpressed by HGSOC and is associated with poor overall survival. Here, we confirm

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