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S2657

Sigma-Aldrich

Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse

clone SH-B4, ascites fluid

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Synonym(s):
Anti-NEF, Anti-S100, Anti-S100-B, Anti-S100beta
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SH-B4, monoclonal

contains

15 mM sodium azide

species reactivity

sheep, bovine, goat, pig, feline, rabbit, rat, canine, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable (: suitable)
indirect ELISA: suitable (:suitable)
western blot: 1:100-1:200 ( (using S-100B Protein, Bovine Brain))

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... S100B(6285)
rat ... S100b(25742)

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This Item
S2532SAB4200671S6797
conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

clone

SH-B4, monoclonal

clone

SH-B1, monoclonal

clone

SH-B1, monoclonal

clone

SH-L1, monoclonal

species reactivity

sheep, bovine, goat, pig, feline, rabbit, rat, canine, human

species reactivity

feline, pig, bovine, sheep, goat, rabbit, canine, human, rat

species reactivity

cat, rabbit, porcine, bovine, rat, human

species reactivity

lizard, goat, rat, bovine, human, feline, frog, canine, pig

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable (: suitable), indirect ELISA: suitable (:suitable), western blot: 1:100-1:200 ( (using S-100B Protein, Bovine Brain))

technique(s)

dot blot: suitable using denatured-reduced preparations, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1000 using protease-digested sections of human tongue, indirect ELISA: suitable, microarray: suitable, radioimmunoassay: suitable

technique(s)

immunohistochemistry: 1.5-3  μg/mL using immunoperoxidase labeling of pronase digested, formalin-fixed, paraffin-embedded sections of rabbit tongue.

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable using native preparations, indirect immunofluorescence: 1:1,000 using cultured Madin-Darby bovine kidney (MDBK) cells, western blot: 1:1,000

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General description

Monoclonal Anti-S-100 (b-Subunit) (mouse IgG1 isotype) is derived from the SH-B4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. S-100 is a set of small, highly acidic, thermolabile dimer proteins of ~20 kDa, widely distributed in different tissues.
S-100 (β-Subunit) is a calcium-binding protein, that belongs to S100 family of calcium-binding proteins. It is expressed in melanocytes. It is located on human chromosome 21q22.3.

Specificity

Recognizes an epitope located on the β chain (i.e. in S-100a and S-100b) but not on the α chain of S-100 (i.e. in S-100a and S-100ao). In ELISA, recognition of S-100 subunit by clone SH-B4 is Ca2+-dependent. The product does not react with other members of the EF-hand family such as, calmodulin, parvalbumin, intestinal calcium-binding protein and myosin light chain. In immunohistochemistry, the antibody detects normal and neoplastic S-100 β subunit-containing cells (e.g. Schwann cells, chondrocytes, melanocytes, and melanotic tumors) in protease-digested, formalin-fixed, paraffin-embedded tissues.

Immunogen

bovine brain S-100b

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse has been used in immunocytochemistry and immunohistochemistry. It has also been used as a component in serum medium for 5-ethynyl-29-deoxyuridine (EdU), incorporation assay.

Biochem/physiol Actions

S-100 (β-Subunit) acts as a marker of melanocyte cytotoxicity. Reduced expression of S100B is observed in chronic liver diseases. It also acts as a serum marker in endocrine resistant breast cancer.
S-100 is considered to be a cell-growth regulator, but other functions have been suggested, e.g., increasing the membrane permeability to cations under physiologic conditions, stimulation of nucleolar RNA polymerase activity, interaction with the tumor suppressor protein p53 and as a carrier of proteins and free fatty acids in adipocytes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Customers Also Viewed

S100 Proteins As an Important Regulator of Macrophage Inflammation
Xia C, et al.
Frontiers in Immunology (2017)
C Spang et al.
Journal of musculoskeletal & neuronal interactions, 15(2), 197-206 (2015-06-03)
The plantaris tendon is increasingly recognised as an important factor in midportion Achilles tendinopathy. Its innervation pattern is completely unknown. Plantaris tendons (n=56) and associated peritendinous tissue from 46 patients with midportion Achilles tendinopathy and where the plantaris tendon was
Amyloid beta-Peptide Directly Induces Spontaneous Calcium Transients, Delayed Intercellular Calcium Waves and Gliosis in Rat Cortical Astrocytes
Chow SK, et al.
ASN Neuro, 2(1) (2010)
Marked innervation but also signs of nerve degeneration in between the Achilles and plantaris tendons and presence of innervation within the plantaris tendon in midportion Achilles tendinopathy
Spang C, et al.
Journal of musculoskeletal & neuronal interactions, 15(2), 197-197 (2015)
S100B promotes injury-induced vascular remodeling through modulating smooth muscle phenotype
Cao T, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1863(11), 2772-2782 (2017)

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