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S3194

Sigma-Aldrich

Stemline® Neural Stem Cell Expansion Medium

without growth factors and antibiotics, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
NSC Medium
NACRES:
NA.81

Quality Level

sterility

sterile-filtered

form

liquid

drug control

regulated under CDSA - not available from Sigma-Aldrich Canada

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

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Stemline® XF MSC Basal Medium Serum-free, liquid, sterile-filtered, suitable for cell culture

SAFC

14371C

Stemline® XF MSC Basal Medium

Stemline® XF MSC Supplement Serum-free, liquid, sterile-filtered, suitable for cell culture

SAFC

14372C

Stemline® XF MSC Supplement

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

400

Quality Level

300

Quality Level

500

Quality Level

500

General description

Stemline® Neural Stem Cell Expansion Medium is an animal component-free formulation optimized specifically for the expansion of human neural stem cells, which retain their differentiative capacity. Stemline Neural Stem Cell Expansion Medium outperforms standard serum-free media formulations for the generation of neural stem cells, without the use of any animal-derived components or the need for additional supplements, other than growth factors.

Application

Stemline® Neural Stem Cell Expansion Medium has been used to expand human neural progenitor cells (hNPCs), human embryonic stem (hES) and induced pluripotent stem (iPS) cells in to free floating spheres.

Components

Stemline® Neural Stem Cell Expansion Medium is a proprietary formulation, which does not contain growth factors, antibiotics, or animal-derived components.

Legal Information

Stemline is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Derivation of Myogenic Progenitors Directly From Human Pluripotent Stem Cells Using a Sphere?Based Culture.
Hosoyama T, et al.
Stem Cells Translational Medicine, 3(5), 564-574 (2014)
Fusako Sakai-Takemura et al.
Communications biology, 3(1), 182-182 (2020-04-22)
Understanding the signaling pathways that regulate proliferation and differentiation of muscle progenitors is essential for successful cell transplantation for treatment of Duchenne muscular dystrophy. Here, we report that a γ-secretase inhibitor, DAPT (N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenylglycine tertial butyl ester), which inhibits the release
Eileen Lynch et al.
Disease models & mechanisms, 12(8) (2019-08-24)
Amyotrophic lateral sclerosis (ALS) is a late-onset neuromuscular disease with no cure and limited treatment options. Patients experience a gradual paralysis leading to death from respiratory complications on average only 2-5 years after diagnosis. There is increasing evidence that skeletal muscle
Fusako Sakai-Takemura et al.
Scientific reports, 8(1), 6555-6555 (2018-04-28)
Human induced pluripotent stem cells (hiPSCs) are a potential source for cell therapy of Duchenne muscular dystrophy. To reliably obtain skeletal muscle progenitors from hiPSCs, we treated hiPS cells with a Wnt activator, CHIR-99021 and a BMP receptor inhibitor, LDN-193189
Ferumoxytol labeling of human neural progenitor cells for diagnostic cellular tracking in the porcine spinal cord with magnetic resonance imaging.
Lamanna J J, et al.
Stem Cells Translational Medicine, 6(1), 139-150 (2017)

Articles

Neural Stem Cell FAQs

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

Protocols

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

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