SAB4200602

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal

~1.0 mg/mL, clone 5C5, purified from hybridoma cell culture

• Synonym(s): Anti-AA959943, Anti-ACTA1, Anti-ALPHA SKELETAL ACTIN, Anti-ALPHA1 ACTIN, Anti-ASMA, Anti-Acta-2, Anti-Acts, Anti-Actsk-1, Anti-CFTD, Anti-CFTDM, Anti-Fetal isoform of skeletal alpha-actin, G-actin, Anti-MPFD, Anti-NEM1, Anti-RP5-1068B5.2, Anti-actin, alpha 1, skeletal muscle
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: 5C5, monoclonal
• Species reactivity: human, carp, bovine, frog, sheep, rabbit, rat, snake, guinea pig
• Application: ELISA (i), IHC
• Citations: 5

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 5C5, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~42 kDa
• species reactivity: human, carp, bovine, frog, sheep, rabbit, rat, snake, guinea pig
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: suitable, immunohistochemistry: 1-2 μg/mL using formalin-fixed, paraffin embedded human tongue, indirect ELISA: suitable
• isotype: IgM
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ACTA1(58)

Description

General description

Monoclonal Anti-Actin (α-Sarcomeric) (mouse IgM isotype) is derived from the hybridoma 5C5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rabbit striated muscle. Actin is one of the major cytoskeletal proteins in eukaryotic cells.

Immunogen

purified rabbit striated muscle

Application

Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse has been used in:

• western blotting
• enzyme linked immunosorbent assay (ELISA)
• immunohistochemistry

Biochem/physiol Actions

Actin plays an essential role in various cellular processes including cell migration, cytokinesis, vesicle transport, and contractile force generation. Pathological conditions influenced by genetic and/or environmental factors causes variations in the sarcomeric actin filaments. These variations in the actin filaments leads to the development of congenital myopathy, a genetic muscle disorder.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable