Merck
All Photos(1)

Documents

SAB4200671

Sigma-Aldrich

Anti-S-100 (β-Subunit) antibody, Mouse monoclonal

clone SH-B1, purified from hybridoma cell culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse, NEF, S100, S100-B, S100beta
NACRES:
NA.46

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

SH-B1, monoclonal

species reactivity

cat, rabbit, porcine, bovine, rat, human

concentration

~1 mg/mL

technique(s)

immunohistochemistry: 1.5-3  μg/mL using immunoperoxidase labeling of pronase digested, formalin-fixed, paraffin-embedded sections of rabbit tongue.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... S100B(6285)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
S2657SAB4200504SAB4200503
antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

clone

SH-B1, monoclonal

clone

SH-B4, monoclonal

clone

SND1-3, monoclonal

clone

1L4, monoclonal

species reactivity

cat, rabbit, porcine, bovine, rat, human

species reactivity

sheep, bovine, goat, pig, feline, rabbit, rat, canine, human

species reactivity

human, rat, mouse

species reactivity

human, rat, mouse

concentration

~1 mg/mL

concentration

-

concentration

~1.0 mg/mL

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry: 1.5-3  μg/mL using immunoperoxidase labeling of pronase digested, formalin-fixed, paraffin-embedded sections of rabbit tongue.

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable (: suitable), indirect ELISA: suitable (:suitable), western blot: 1:100-1:200 ( (using S-100B Protein, Bovine Brain))

technique(s)

western blot: 1-2 μg/mL using whole extracts of human HeLa or HEK-293T cells.

technique(s)

indirect immunofluorescence: 5-10 μg/mL using Human HeLa cells., western blot: 0.5-1 μg/mL using Whole extracts of mouse Hepa1-6 cells.

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Monoclonal Anti-S-100 (β-subunit) (mouse IgG1 isotype) is derived from the SH-B1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. S-100 is a set of small, thermolabile, highly acidic homo or hetero-dimer calcium binding proteins. The protein exists in two isoforms namely, S-100α and S-100β, which are brain specific.
S-100β is a calcium binding protein. It is mainly present in astrocytes and neurons of hindbrain and spinal cord.

Immunogen

Purified bovine brain S-100β

Application

Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse has been used in:
  • immunohistochemistry
  • enzyme linked immunosorbent assay (ELISA) (Ca2+ ion independent)
  • immunocytochemistry
  • immunoblotting
  • dot blot
  • immunohistochemistry.

Biochem/physiol Actions

S-100 is involved in cell-growth regulation, increasing membrane permeability to cations, inflammatory response in many brain diseases, including schizophrenia, stimulation of nucleolar RNA polymerase activity and transporting proteins and free fatty acids in adipocytes. S-100β tissue distribution can be a useful tool in the differential diagnosis of neoplasms and proliferative processes.
S-100β protein interacts with synaptic, cytoskeletal and cell cycle proteins. Additionally, it can regulate calcium levels in glial and neuronal cells. It is involved in neuronal plasticity, astrogliosis and neuronal cell survival. S-100β is associated with Alzheimer disease and amyotrophic lateral sclerosis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

A Migheli et al.
Neuroscience letters, 261(1-2), 25-28 (1999-03-19)
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by progressive motor neuron loss and astrogliosis. We studied the immunohistochemical expression of S-100beta, a calcium-binding protein with both neurotrophic and neurotoxic activities, in the spinal cord of patients with ALS.
Postnatal activation of TLR4 in astrocytes promotes excitatory synaptogenesis in hippocampal neurons
Shen Y, et al.
The Journal of cell biology, 215(5), 719-734 (2016)
Marc Oria et al.
Frontiers in molecular neuroscience, 15, 888351-888351 (2022-07-06)
During embryonic spinal cord development, neural progenitor cells (NPCs) generate three major cell lines: neurons, oligodendrocytes, and astrocytes at precise times and locations within the spinal cord. Recent studies demonstrate early astrogenesis in animal models of spina bifida, which may
Clinicoradiological characteristics, management and prognosis of primary myeloid sarcoma of the central nervous system: A report of four cases.
Yang B, et al.
Oncology Letters, 14(3), 3825-3831 (2017)
Yi Shen et al.
The Journal of cell biology, 215(5), 719-734 (2016-12-07)
Astrocytes are critical in synapse development, and their dysfunction in crucial developmental stages leads to serious neurodevelopmental diseases, including seizures and epilepsy. Immune challenges not only affect brain development, but also promote seizure generation and epileptogenesis, implying immune activation is

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service