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MISSION® shRNA Product Offerings

Order Custom and Predesigned shRNA

High-throughput shRNA, shRNA library, targeted integration shRNA, Small hairpin RNA, Short hairpin RNA, shRNA, shRNA gene sets, shRNA panels, MISSION® shRNA, inducible shRNA, shRNA arrayed, Custom shRNA, shRNA pools, RNAi

MISSION® shRNA Products

Individual MISSION® shRNA Clones

  • Our online tool allows you to view clone-specific validation information and build your own clone sets.
  • The individual clones are available in three formats: bacterial glycerol stock, purified plasmid DNA and lentiviral transduction particles
  • Locate shRNA clones for your specific gene of interest by clicking 'Search'
  • Choose from over 315,000 pre-cloned shRNA constructs for both human and mouse.
  • Learn More




MISSION® shRNA Gene Family Sets

Choose from 25 Pre-Designed shRNA Gene Panels

  • All TRC shRNA clones have been methodically mapped to each current gene set by our Bioinformatics team.
  • Gene family sets for human (22) and mice (3) include popular kinases, phosphatases, GPCRs, tyrosine kinases, and epigenetic regulators.
  • Any panel can be arrayed in either 96- or 384- well plate formats
  • All gene family sets can be used at the benchtop for focused target discovery and pathway analysis.




Create your own panel.
Different gene pathway of interest? Want a more complete list?
Submit a request.

  • Gene family sets are fully customizable allowing for complete flexibility to add or delete clone or gene content.

MISSION® shRNA Libraries

Arrayed genome-wide shRNA libraries

  • Most extensive coverage: Pre-cloned genome-wide arrayed libraries contain 245,000+ clone shRNA library targeting 20,000+ human and 21,000+ mouse genes.
  • High-throughput manufacturing: World class high-throughput viral manufacturing.
  • Flexible format options: Entire libraries or single gene sets offered in glycerol stock, DNA, or viral format.
  • Extended Coverage=Average of 5 - 6 clones targeting each gene.



Pooled shRNA Libraries
Whole genome shRNA screening for a fraction of the cost.

  • Affordable - Bench top discovery without robotics or liquid handling.
  • Comprehensive - Trusted TRC (The RNAi Consortium) content with shRNA taregting 20,000+ human or 21,000+ mouse genes including TRC 1.5 and 2.0 (unique and validated) add-ons which are exclusive to Sigma.
  • Universal - Simplified whole genome RNAi screening in standard or difficult to expand cell lines.
  • Versatile - Compatible with FACS and most downstream fluorescent assays.



Fully customizable shRNA sequence, vector, and lentiviral manufacturing

  • Select by methods other than puromycin, including neomycin (G418) and FACS via reporters.
  • Confirm your lentiviral delivery through a fluorescent reporter.
  • Customize your shRNA sequence.
  • Eliminate tedious cloning and viral production.
  • Service includes: shRNA design, cloning, sequence verifications, DNA quantitation, and titer determination.

Custom Capabilities: Bringing you more flexibility

  • Contract manufacturing: Send us your clones for lentiviral production! We accommodates high-throughput projects, private collections, and alternate libraries. Please request a quote or contact if interested.


Create Your Own shRNA Pool

Focus on the genes essential to your research by creating your own custom shRNA pool

  • Create your custom shRNA pool for maximum return of relevant hits
  • Customize your volume and aliquoting needs to further enhance your ability to rapidly screen multiple cell lines.
  • Titers and volumes adequate for in vitro, in vivo and xenograft applications.
  • Partner with Sigma's next-generation sequencing services for data deconvolution and maximum data return from your screening efforts.



Deconvolution of shRNA Pools

  • Sigma's deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.
  • Next-generation sequencing of clones gives a precise number of individual clone occurrence within a pooled shRNA sample.
  • Comprehensive, reproducible results from pooled shRNA screens.
  • Statistically robust and information-rich data.
  • Learn More

shRNA Controls

Measure transduction and knockdown efficiency, or monitor off-target effects.

  • Positive Transduction Controls: Empty Vector, TurboGFP, TagCFP, TagYFP, TagRFP, and TagFP365 Controls
  • Positive Knockdown Controls: TurboGFP, eGFP, luciferase, B2M, and ARHGDIA controls
  • Negative Controls: Empty Vector, Non-target, Turbo GFP, eGFP, and luciferase controls
  • Learn More




Produce high-titer lentivirus using the MISSION® Lentiviral Packaging Mix

  • Accelerate viral production by skipping tedious but critical multivariable plasmid optimization.
  • Produce high titer lentivirus with MISSION® shRNA plasmids with the included easy to use protocol.
  • Safely produce lentivirus utilizing the three plasmid system with self inactivating, long terminal repeat (SIN/LTR) regions.
  • Choose between small or large scale lentivirus production with our conveniently sized 250 µl or 1.7 ml sizes.

Packaging Mix

Inducible MISSION® shRNA

Tight regulation meets great gene silencing

  • IPTG-inducible shRNA pLKO-vectors allow for fast response and temporally-controlled gene silencing useful for essential or lethal gene knockdown.
  • Proven to work in vivo and in vitro
  • Single vector systems in 1X LacO and 3X LacO formats for effective inducible knockdown
  • Available on demand service includes: shRNA design(choose any TRC clone or custom sequence), cloning, sequence verification, DNA quantitation, and titer determination.


Panagiotis Maragozidis et al.
Molecular cancer, 14, 187-187 (2015-11-07)
Lung cancer is the leading cause of cancer mortality worldwide, mainly due to late diagnosis, poor prognosis and tumor heterogeneity. Thus, the need for biomarkers that will aid classification, treatment and monitoring remains intense and challenging and depends on the...
Carole Siret et al.
British journal of cancer, 113(10), 1445-1453 (2015-10-30)
Malignant transformation of melanocytes frequently coincides with an alteration in the expression of cell-cell adhesion molecules (cadherins) and cell-extracellular matrix proteins (integrins). How these two adhesion systems interplay to impact on cell invasion remains to be described in melanoma. Cell...
Plasmids: shopping in the age of plenty.
Vivien Marx
Nature methods, 11(8), 795-798 (2014-07-31)
Hélène Lazareth et al.
Nature communications, 10(1), 3303-3303 (2019-07-26)
The mechanisms driving the development of extracapillary lesions in focal segmental glomerulosclerosis (FSGS) and crescentic glomerulonephritis (CGN) remain poorly understood. A key question is how parietal epithelial cells (PECs) invade glomerular capillaries, thereby promoting injury and kidney failure. Here we...
Y Wang et al.
Oncogene, 35(11), 1363-1372 (2015-08-04)
RNF126 is an E3 ubiquitin ligase. The deletion of RNF126 gene was observed in a wide range of human cancers and is correlated with improved disease-free and overall survival. These data highlight the clinical relevance of RNF126 in tumorigenesis and...

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