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  • SRP0129
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SRP0129

Sigma-Aldrich

Dot1L human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

All Photos(1)
Synonym(s):
DOT1-like, histone H3 methyltransferase, H3-K79-HMTase, KIAA1814, KMT4, Lysine N-methyltransferase 4
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

73 kDa

packaging

pkg of 50 μg

concentration

>0.02 mg/mL

NCBI accession no.

NM_032482

UniProt accession no.

Q8TEK3

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... DOT1L(84444)

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Dot1L human recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

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Sigma-Aldrich

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biological source

human

biological source

human

biological source

-

biological source

-

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

assay

≥70% (SDS-PAGE)

assay

≥90% (HPLC), ≥90% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

form

aqueous solution

form

lyophilized

form

lyophilized

form

lyophilized powder

mol wt

73 kDa

mol wt

24.4 kDa

mol wt

19.6 kDa

mol wt

calculated mol wt 19.9 kDa (with Gly-Pro at the N- terminus), observed mol wt 35-45 kDa (DTT-reduced.Ser 23 is the predicted N-terminus .)

packaging

pkg of 50 μg

packaging

pkg of 25 μg

packaging

-

packaging

-

General description

Human DOT1L, GenBank Accession No. NM_032482), amino acids 2-416 with N-terminal ST tag, MW = 73 kDa, expressed in E. coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 7.5, 100mM NaCl, 0.05% Tween 20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Certificate of Analysis

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Certificate of Origin

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