After reconstituting the protein in water, it remains active and stable at 2-8°C for up to a week. However, for long-term stabilization at -20 to -80°C, further dilution with a buffer containing a carrier is necessary to prevent activity loss. It is recommended to use 5% trehalose or 0.1% BSA in water or PBS for extended storage. Working aliquots should have a minimum volume of 10 uL and a concentration above 1 ug/ml for optimal stability.
SRP3027
EGF human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
Epidermal Growth Factor, URG, Urogastrone
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500 μG
£425.00
About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
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biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC), ≥98% (SDS-PAGE)
form
lyophilized
potency
≤0.2 ng/mL
mol wt
6.2 kDa
packaging
pkg of 500 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... EGF(1950)
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1 of 4
This Item | |||
|---|---|---|---|
| assay ≥98% (HPLC), ≥98% (SDS-PAGE) | assay ≥97% (SDS-PAGE) | assay ≥98% (HPLC), ≥98% (SDS-PAGE) | assay ≥97% (HPLC), ≥97% (SDS-PAGE) |
| biological source human | biological source human | biological source human | biological source human |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in CHO cells |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | embryo: suitable |
| form lyophilized | form lyophilized powder | form lyophilized | form lyophilized |
| packaging pkg of 500 μg | packaging pkg of 5X0.2 mg, pkg of 0.2 mg, pkg of 0.5 mg | packaging pkg of 10 μg | packaging pkg of 1 mg, pkg of 10 μg |
General description
The gene EGF (epidermal growth factor) is mapped to human chromosome 4q25-27.[1] The EGF gene encodes for a large type I membrane–anchored precursor protein which is cleaved into pro-EGF for generation of peptide hormone EGF. The mRNA is expressed in kidney, salivary gland, cerebrum and prostate.[2] Recombinant human EGF is a 6.2kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds.
Application
EGF (epidermal growth factor) human has been used in the culture medium for the maintenance of MCF10A cells (human mammary epithelial cells).[2]
Biochem/physiol Actions
EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells.[3] Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing.[4][5] EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor.[6] This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). Mutation in the EGF gene might be associated with risk of non- small cell lung adenocarcinoma.[1] It is also involved in anaplastic thyroid cancer and renal hypomagnesemia. It controls cell differentiation and neurotrophic processes.[7][8]
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Other Notes
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Find documentation for the products that you have recently purchased in the Document Library.
Basal p21 controls population heterogeneity in cycling and quiescent cell cycle states.
Overton KW
Proceedings of the National Academy of Sciences of the USA, 111, E4386-E4393 (2014)
Neurotrophic actions of a novel molluscan epidermal growth factor.
Hermann PM
The Journal of Neuroscience, 20, 6355-6364 (2000)
The miR-200 family regulates the epithelial-mesenchymal transition induced by EGF/EGFR in anaplastic thyroid cancer cells.
Zhang Z
International Journal of Molecular Medicine, 30, 856-862 (2012)
Clinical Implication of EGF A61G Polymorphism in the Risk of Non Small Cell Lung Adenocarcinoma Patients: A Case Control Study.
Masroor M
Asian Pacific Journal of Cancer Prevention, 16, 7529-7534 (2015)
Transforming growth factor alpha and epidermal growth factor in protection and healing of gastric mucosal injury.
Konturek SJ
Scandinavian Journal of Gastroenterology, 27, 649-655 (1992)
Articles
Discover answers to your frequently asked questions about animal component-free, xenobiotic-free, and chemically defined media and reagents for cell culture.
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Is it necessary to dilute item no: SRP3027-500UG in a buffer with a stabilizer for extended storage as mentioned in the reconstitution portion of the specification. If so, would D-(+)-Trehalose dihydrate (cat#T0167) be appropriate? Also, specify the buffer considering that water is indicated as an appropriate diluent in the specifications.
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