SRP6033

LBP human

recombinant, expressed in CHO cells, 90-95% (SDS-PAGE)

• Synonym(s): Lipopolysaccharide-binding protein
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in CHO cells
• Assay: 90-95% (SDS-PAGE)
• form: lyophilized
• mol wt: 58.0 kDa
• packaging: pkg of 10 μg
• technique(s): ELISA: suitable
• impurities: Endotoxin, tested
• NCBI accession no.: P18428
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... LBP(3929)

Description

General description

Natural lipopolysaccharide binding protein (LBP) is a 58kDa glycoprotein produced in liver. This is a His-tagged protein. It is part of a family of lipid-binding proteins. The gene encoding this protein is localized on human chromosome 20q11.23.

Biochem/physiol Actions

Lipopolysaccharide binding protein (LBP) binds at lipid A of lipopolysaccharide (LPS) with high affinity (10-9M) and reduces the cellular LPS effects at CD14⁺ cells (IL1β, IL6, TNF-α). It acts as opsonin for GRAM negative cells, LPS, neutrophils and granulocytes. It transfers LPS to its receptor and this leads to an active inflammatory response. LBP also binds to LPS-coated erythrocytes. The protein has a role in innate immune responses against Gram-negative bacterial infections.

Physical form

Lyophilized from a concentrated protein solution (0.25 mg/ml) containing PBS, pH 7.1

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile distilled H?O or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable